Jeske Y W, Bowles J, Greenfield A, Koopman P
Centre for Molecular and Cellular Biology, University of Queensland, Brisbane, Australia.
Nat Genet. 1995 Aug;10(4):480-2. doi: 10.1038/ng0895-480.
Sry is the Y-chromosomal gene that is pivotal in the determination of sex in mammals, however the structure of the Sry transcript produced in the embryo has not been determined. We show here that the transcript expressed in the developing mouse gonad at the sex determining stage of development is linear, polyadenylated and encoded by a single exon, in contrast to the circular, apparently untranslated transcript produced in adult testes. The linear transcript was not detected in any other fetal tissue nor in any adult tissue tested, and was expressed only in the genital ridge portion of the urogenital ridge. The spatial and temporal profile of Sry expression suggests that its role in the mouse fetus is limited to initiating Sertoli cell development during testis determination.
Sry是Y染色体上的基因，在哺乳动物性别决定中起关键作用，然而胚胎中产生的Sry转录本的结构尚未确定。我们在此表明，在发育的性别决定阶段，发育中的小鼠性腺中表达的转录本是线性的、多聚腺苷酸化的，且由单个外显子编码，这与成年睾丸中产生的环状、明显未翻译的转录本形成对比。在任何其他胎儿组织或所检测的任何成年组织中均未检测到线性转录本，且其仅在泌尿生殖嵴的生殖嵴部分表达。Sry表达的时空模式表明，其在小鼠胎儿中的作用仅限于在睾丸决定过程中启动支持细胞的发育。
