Sheu S J, Lu C F
Department of Chemistry, National Taiwan Normal University, Taipei.
J Chromatogr A. 1995 Jun 9;704(2):518-23. doi: 10.1016/0021-9673(95)00216-a.
A high-performance liquid chromatographic method for the simultaneous determination of eight constituents (gallic acid, sennoside B, sennoside A, naringin, hesperidin, honokiol, magnolol and emodin) of the Chinese herbal formula hsiao-cheng-chi-tang was established. Various samples of the formula were separated using a Cosmosil 5C18 column with a linear gradient elution system consisting of acetate buffer as mobile phase. Contents of these marker substances in an unpretreated hsiao-cheng-chi-tang extract could be easily determined within 60 min. The effects of pH, buffer concentration and column selectivity for this method are described.
建立了一种高效液相色谱法，用于同时测定中药方剂小承气汤中的8种成分（没食子酸、番泻苷B、番泻苷A、柚皮苷、橙皮苷、厚朴酚、和厚朴酚及大黄素）。使用Cosmosil 5C18柱，以醋酸盐缓冲液作为流动相的线性梯度洗脱系统对该方剂的各种样品进行分离。未预处理的小承气汤提取物中这些标志性物质的含量可在60分钟内轻松测定。描述了该方法中pH值、缓冲液浓度和柱选择性的影响。
