Suzuki K T, Ogra Y
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Res Commun Mol Pathol Pharmacol. 1995 May;88(2):187-95.
Copper (Cu) accumulating in the liver of LEC rats (Long-Evans rats with a cinnamon-like coat color) is bound to metallothionein (MT). Mechanisms for the removal of Cu by tetrathiomolybdate (TTM) were studied by the high performance liquid chromatography/inductively coupled plasma--mass spectrometry (HPLC/ICP-MS) method. MT containing Cu and cadmium (Cd) (Cu,Cd-MT) was reacted with TTM at a molar ratio of TTM/Cu = 0.5. A complex containing Cu, Cd and molybdenum (Mo) was formed and migrated to a position corresponding to an MT dimer on a gel filtration column. This complex designated previously as a dimer of MT through -S-Cu-S- bridge was revised to be a complex formed between MT and TTM through (MT)-S-Cu-S-(TTM) bridge with differing numbers of TTM bound to MT.
在LEC大鼠（具有类似肉桂色皮毛的Long-Evans大鼠）肝脏中积累的铜（Cu）与金属硫蛋白（MT）结合。采用高效液相色谱/电感耦合等离子体质谱（HPLC/ICP-MS）法研究了四硫代钼酸盐（TTM）去除铜的机制。含铜和镉（Cd）的MT（Cu,Cd-MT）与TTM以TTM/Cu = 0.5的摩尔比反应。形成了一种包含铜、镉和钼（Mo）的络合物，并在凝胶过滤柱上迁移到与MT二聚体相对应的位置。这种先前通过-S-Cu-S-桥被指定为MT二聚体的络合物被修正为通过（MT）-S-Cu-S-（TTM）桥在MT和TTM之间形成的络合物，其中与MT结合的TTM数量不同。
