Hao H, Hirota S, Tsukamoto Y, Imakita M, Ishibashi-Ueda H, Yutani C
Department of Pathology, National Cardiovascular Center, Suita, Japan.
Arterioscler Thromb Vasc Biol. 1995 Sep;15(9):1474-80. doi: 10.1161/01.atv.15.9.1474.
We examined the expression of matrix Gla protein (MGP), osteopontin (OPN), and osteonectin (ON) mRNAs in aortic rings excised from 3-month-, 10-month-, and 2-week-old rats during 72-hour incubations in serum-free media. In the aortic rings from 3-month-old rats, the expression of MGP mRNA was strong before incubation and increased during the 72-hour incubation. The expression of OPN mRNA was first detected after a 5-hour incubation and increased thereafter, and that of ON mRNA was strong before the incubation and decreased during the incubation. The expression of MGP and OPN mRNAs in 10-month- and 2-week-old rats was similar to that in 3-month-old rats. In contrast, expression of ON mRNA in 10-month-old rats and the expression of ON mRNA in 2-week-old rats was stronger than that in 3-month-old rats at every incubation period. In situ hybridization and immunohistochemistry identified the MGP, OPN, and ON mRNA-expressing cells as vascular smooth muscle cells. These results suggest that the expression of these mRNAs was regulated in incubation time-dependent and age-specific ways. We believe that this organ culture model is useful for further studies of the function of these bone matrix proteins and regulation of their expression in the vessel wall.
我们检测了从3月龄、10月龄和2周龄大鼠切除的主动脉环在无血清培养基中孵育72小时期间基质Gla蛋白（MGP）、骨桥蛋白（OPN）和骨连接蛋白（ON）mRNA的表达。在3月龄大鼠的主动脉环中，MGP mRNA的表达在孵育前很强，并在72小时孵育期间增加。OPN mRNA的表达在孵育5小时后首次检测到，此后增加，而ON mRNA的表达在孵育前很强，在孵育期间下降。10月龄和2周龄大鼠中MGP和OPN mRNA的表达与3月龄大鼠相似。相反，在每个孵育期，10月龄大鼠ON mRNA的表达和2周龄大鼠ON mRNA的表达均强于3月龄大鼠。原位杂交和免疫组织化学鉴定出表达MGP、OPN和ON mRNA的细胞为血管平滑肌细胞。这些结果表明，这些mRNA的表达以孵育时间依赖性和年龄特异性方式受到调节。我们认为，这种器官培养模型有助于进一步研究这些骨基质蛋白的功能及其在血管壁中表达的调节。
