Hirakawa K, Hirota S, Ikeda T, Yamaguchi A, Takemura T, Nagoshi J, Yoshiki S, Suda T, Kitamura Y, Nomura S
Department of Pathology, Osaka University Medical School, Suita, Japan.
J Bone Miner Res. 1994 Oct;9(10):1551-7. doi: 10.1002/jbmr.5650091007.
The expression of the mRNAs for osteonectin (ON), osteopontin (OPN), osteocalcin (OC), and matrix Gla protein (MGP) was studied by in situ hybridization during the healing process of an experimental fracture in adult rat femora. At day 1 postoperatively, ON mRNA was detected in the proliferating periosteum. At day 3, ON, OPN, and OC mRNAs were detected in woven bone. From day 5, MGP and ON mRNAs were detected in the immature chondrocytes. From day 7, ON, OPN, and OC mRNAs were detected in the osteoblastic cells in newly formed endosteal trabecular bone. OPN mRNA was also detected in some of the osteocytes in trabecular bone. From day 14, OPN and MGP mRNAs were detected in newly formed periosteal hypertrophic chondrocytes, and the ON, OPN, and OC mRNAs were detected in osteoblastic cells in newly formed periosteal trabecular bone. Although the cell types that expressed each mRNA in fractured bones were similar to those in embryonic bones, the time course of these mRNA expression in fractured bones was different from that in embryonic bones. We considered that this system is useful to investigate the phenotypic change in osteogenic and chondrogenic lineage cells that appears during fracture healing at the molecular level.
采用原位杂交技术，研究成年大鼠股骨实验性骨折愈合过程中骨连接蛋白（ON）、骨桥蛋白（OPN）、骨钙素（OC）和基质Gla蛋白（MGP）mRNA的表达情况。术后第1天，在增殖的骨膜中检测到ON mRNA。第3天，在编织骨中检测到ON、OPN和OC mRNA。从第5天开始，在未成熟软骨细胞中检测到MGP和ON mRNA。从第7天开始，在新形成的骨内膜小梁骨的成骨细胞中检测到ON、OPN和OC mRNA。在小梁骨的一些骨细胞中也检测到OPN mRNA。从第14天开始，在新形成的骨膜肥大软骨细胞中检测到OPN和MGP mRNA，在新形成的骨膜小梁骨的成骨细胞中检测到ON、OPN和OC mRNA。尽管骨折骨中表达每种mRNA的细胞类型与胚胎骨中的相似，但这些mRNA在骨折骨中的表达时间进程与胚胎骨中的不同。我们认为该系统有助于在分子水平上研究骨折愈合过程中出现的成骨和软骨形成谱系细胞的表型变化。
