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[牛和山羊培养的输卵管上皮细胞中半胱氨酸亚磺酸盐脱羧酶(EC 4.1.1.29)的证明]

[Demonstration of cysteine sulfinate decarboxylase (EC 4.1.1.29) in cultured oviduct epithelial cells in cows and goats].

作者信息

Guérin P, Tappaz M, Guillaud J, Menezo Y

机构信息

ENV Lyon BP 83, Marcy-L'Etoile, France.

出版信息

C R Acad Sci III. 1995 May;318(5):523-8.

PMID:7670997
Abstract

Tubal fluid contains high amounts of hypotaurine and taurine. These amino-acids are important for gametes and embryo survival. They are synthesized and secreted by oviduct epithelial cells in vitro. Cysteine sulfinate decarboxylase (EC 4.1.1.29) activity was identified by selective immuno-trapping using a specific antiserum in cow and goat oviduct epithelial monolayers. This result suggests that cysteine is converted to hypotaurine and taurine via cysteine sulfinic acid in these cells.

摘要

输卵管液中含有大量的亚牛磺酸和牛磺酸。这些氨基酸对配子和胚胎的存活很重要。它们在体外由输卵管上皮细胞合成和分泌。在牛和山羊的输卵管上皮单层细胞中,通过使用特异性抗血清的选择性免疫捕获鉴定了半胱氨酸亚磺酸脱羧酶(EC 4.1.1.29)的活性。这一结果表明,在这些细胞中,半胱氨酸通过半胱氨酸亚磺酸转化为亚牛磺酸和牛磺酸。

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