Nakao M, Yamana H, Imai Y, Toh Y, Toh U, Kimura A, Yanoma S, Kakegawa T, Itoh K
Department of Immunology, Kurume University School of Medicine, Japan.
Cancer Res. 1995 Oct 1;55(19):4248-52.
The CD4-CD8+ CTL (KE-4 CTL) cell line against autologous tumor cells was established in a patient with esophageal cancer. This KE-4 CTL recognized a peptide antigen on esophageal and lung squamous cell carcinomas in an HLA A2601-restricted manner, as evaluated by cytotoxicity against a panel of tumor cells, transfection experiments with HLA A2601 cDNA, and reconstitution with eluted peptides. None of the normal cells tested was lysed by this CTL. These results suggest the existence of HLA A2601-restricted CTL precursors recognizing a peptide antigen on SCC in a patient with esophageal cancer.
在一名食管癌患者中建立了针对自体肿瘤细胞的CD4-CD8+CTL（KE-4 CTL）细胞系。通过对一组肿瘤细胞的细胞毒性、HLA A2601 cDNA转染实验以及洗脱肽的重组实验评估，该KE-4 CTL以HLA A2601限制性方式识别食管和肺鳞状细胞癌上的一种肽抗原。所测试的正常细胞均未被该CTL裂解。这些结果表明，在一名食管癌患者中存在识别鳞状细胞癌上肽抗原的HLA A2601限制性CTL前体。
