Iguchi I, Kamiyama K, Wang X, Kita M, Imanishi J, Yamaguchi N, Sotozono C, Kinoshita S
Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
Cornea. 1995 Jul;14(4):365-71. doi: 10.1097/00003226-199507000-00004.
To investigate the role of platelet-derived growth factor (PDGF) in corneal wound healing, we examined the effect of human natural PDGF, recombinant PDGF-BB, and PDGF-AA on the migration of rabbit corneal endothelial cells. In a modified Boyden chamber in fetal bovine serum-free conditions, natural PDGF and PDGF-BB, at a concentration of 1-3 or 10 ng/ml, enhanced the migration of endothelial cells, whereas at a higher concentration (10 or 30 ng/ml), this enhanced migration was suppressed; the optimal concentration range for enhancing migration was 3-10 ng/ml. PDGF-AA did not enhance the migration. Natural PDGF and PDGF-BB activity was found for up to 6 h after the beginning of culture, and was completely blocked by anti-PDGF neutralizing antibodies. A checkerboard assay demonstrated that PDGF-BB had a chemotactic effect on the corneal endothelial cell migration. These results suggest that natural PDGF and PDGF-BB, but not PDGF-AA, are involved in corneal wound healing by stimulating the migration of corneal endothelial cells.
为了研究血小板衍生生长因子（PDGF）在角膜伤口愈合中的作用，我们检测了人天然PDGF、重组PDGF-BB和PDGF-AA对兔角膜内皮细胞迁移的影响。在改良的Boyden小室中，于无胎牛血清条件下，浓度为1 - 3或10 ng/ml的天然PDGF和PDGF-BB可增强内皮细胞的迁移，而在更高浓度（10或30 ng/ml）时，这种增强的迁移受到抑制；增强迁移的最佳浓度范围为3 - 10 ng/ml。PDGF-AA未增强迁移。在培养开始后长达6小时可检测到天然PDGF和PDGF-BB的活性，且其活性可被抗PDGF中和抗体完全阻断。棋盘分析表明，PDGF-BB对角膜内皮细胞迁移具有趋化作用。这些结果提示，天然PDGF和PDGF-BB而非PDGF-AA通过刺激角膜内皮细胞迁移参与角膜伤口愈合。
