Kamiyama K, Iguchi I, Wang X, Imanishi J
Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
Cornea. 1998 May;17(3):315-25.
We investigated the effects of human platelet-derived growth factor (PDGF) isomers on the migration of rabbit corneal fibroblasts and epithelial cells in the presence or absence of fibronectin (FN).
A modified Boyden's chamber method was used to detect cell migration. Cells placed in the inner chamber were incubated with PDGF in the outer chamber at 37 degrees C at 24 h. In addition, epithelial cells were incubated in the presence or absence of FN. The migrated cells were stained and the cell number counted under a microscope. Checkerboard analysis was used to distinguish chemotaxis from chemokinesis.
Natural PDGF and PDGF-BB enhanced the migration of corneal fibroblasts, whereas PDGF-AA did not. As for corneal epithelial cells, all the isomers of PDGF enhanced the migration, but only in the presence of FN, as in the absence of FN or at a low concentration gradient of FN, there was no enhanced cell migration. A checkerboard assay demonstrated that PDGF-BB had a chemotactic effect on the migration of corneal fibroblasts and epithelial cells, whereas PDGF-AA had a chemotactic effect on only corneal epithelial cells.
These results suggest that PDGF is involved in corneal wound healing by stimulating the migration of corneal epithelial cells in the presence of FN and fibroblasts.
我们研究了在有或没有纤连蛋白（FN）存在的情况下，人血小板衍生生长因子（PDGF）异构体对兔角膜成纤维细胞和上皮细胞迁移的影响。
采用改良的Boyden室法检测细胞迁移。将置于内室的细胞与外室中的PDGF在37℃孵育24小时。此外，上皮细胞在有或没有FN的情况下进行孵育。对迁移的细胞进行染色，并在显微镜下计数细胞数量。采用棋盘分析来区分趋化性和趋动性。
天然PDGF和PDGF-BB增强了角膜成纤维细胞的迁移，而PDGF-AA则没有。至于角膜上皮细胞，所有PDGF异构体都增强了迁移，但仅在有FN存在的情况下，因为在没有FN或FN浓度梯度较低时，细胞迁移没有增强。棋盘分析表明，PDGF-BB对角膜成纤维细胞和上皮细胞的迁移有趋化作用，而PDGF-AA仅对角膜上皮细胞有趋化作用。
这些结果表明，PDGF通过在有FN和成纤维细胞存在的情况下刺激角膜上皮细胞的迁移而参与角膜伤口愈合。
