Landolt R M, Vaughan L, Winterhalter K H, Zimmermann D R
Department of Pathology, University of Zürich, Switzerland.
Development. 1995 Aug;121(8):2303-12. doi: 10.1242/dev.121.8.2303.
Chondroitin sulfate proteoglycans have been implicated in the regulation of cell migration and pattern formation in the developing peripheral nervous system. To identify whether the large aggregating proteoglycan versican might be mediating these processes, we prepared monospecific antibodies against a recombinant core protein fragment of chick versican. The purified antibodies recognize the predominant versican splice-variants V0 and V1. Using these antibodies, we revealed a close correlation between the spacio-temporal expression of versican and the formation of molecular boundaries flanking or transiently blocking the migration pathways of neural crest cells or motor and sensory axons. Versican is present in the caudal sclerotome, the early dorsolateral tissue underneath the ectoderm, the pelvic girdle precursor and to a certain extent in the perinotochordal mesenchyme. Versican is completely absent from tissues invaded by neural crest cells and extending axons. Upon completion of neural crest cell migration and axon outgrowth, versican expression is shifted to pre-chondrogenic areas. Since versican inhibits cellular interactions with fibronectin, laminin and collagen I in vitro, the selective expression of versican within barrier tissues may be linked to a functional role of versican in the guidance of migratory neural crest cells and outgrowing axons.
硫酸软骨素蛋白聚糖与发育中的周围神经系统中细胞迁移和模式形成的调节有关。为了确定大型聚集蛋白聚糖多功能蛋白聚糖是否可能介导这些过程，我们制备了针对鸡多功能蛋白聚糖重组核心蛋白片段的单特异性抗体。纯化的抗体识别主要的多功能蛋白聚糖剪接变体V0和V1。使用这些抗体，我们揭示了多功能蛋白聚糖的时空表达与位于神经嵴细胞或运动和感觉轴突迁移途径两侧或暂时阻断其迁移途径的分子边界的形成之间存在密切相关性。多功能蛋白聚糖存在于尾侧体节、外胚层下方的早期背外侧组织、骨盆带前体以及一定程度上的脊索周围间充质中。神经嵴细胞和延伸轴突侵入的组织中完全没有多功能蛋白聚糖。在神经嵴细胞迁移和轴突生长完成后，多功能蛋白聚糖的表达转移到软骨形成前的区域。由于多功能蛋白聚糖在体外抑制细胞与纤连蛋白、层粘连蛋白和I型胶原的相互作用，多功能蛋白聚糖在屏障组织内的选择性表达可能与多功能蛋白聚糖在引导迁移的神经嵴细胞和生长中的轴突方面的功能作用有关。
