Deller T, Nitsch R
Institute of Anatomy, University of Freiburg, Germany.
Exp Brain Res. 1995;104(2):243-8. doi: 10.1007/BF00242010.
This study describes an approach for disconnecting the septal region from the hippocampus by fimbria-fornix lesions while sparing the commissural projections. After a frontal cut through the rostral fornix, commissural fibres were labelled with the anterograde tracer Phaseolus vulgaris leucoagglutinin. The commissural fibre bundle located in the posterior-basal fornix (ventral hippocampal commissure) remained unaffected by the rostral fornix transection, whereas the absence of septal fibres in the hippocampus could be verified using AChE histochemistry. Thus, using this approach, selective studies of the septo-hippocampal projection can be performed while leaving the overwhelming portion of the commissural fibre system intact.
本研究描述了一种通过穹窿-海马伞损伤将隔区与海马分离的方法，同时保留连合投射。在通过喙侧穹窿进行额部切割后，用顺行示踪剂菜豆白细胞凝集素标记连合纤维。位于后基底穹窿（腹侧海马连合）的连合纤维束不受喙侧穹窿横断的影响，而海马中隔纤维的缺失可通过乙酰胆碱酯酶组织化学进行验证。因此，使用这种方法，可以在保留大部分连合纤维系统完整的情况下，对隔-海马投射进行选择性研究。
