大蒜（葱属植物）鳞茎中的脂氧合酶途径：新型二乙烯基醚氧化脂质的检测

The lipoxygenase pathway in garlic (Allium sativum L.) bulbs: detection of the novel divinyl ether oxylipins.

作者信息

Grechkin A N, Fazliev F N, Mukhtarova L S

机构信息

Institute of Biology, Russian Academy of Sciences, Kazan.

出版信息

FEBS Lett. 1995 Sep 4;371(2):159-62. doi: 10.1016/0014-5793(95)00895-g.

DOI:10.1016/0014-5793(95)00895-g

PMID:7672118

Abstract

Incubations of [1-14C]linoleic acid or [1-14C]-(9Z,11E, 13S)-13-hydropero xy-9,11-octadecadienoic acid (13-HPOD) with juice of garlic bulbs lead to the formation of one predominant labelled product, viz., the novel divinyl ether (9Z,11E, 1'E)-12-(1'-hexenyloxy)-9,11-dodecadienoic acid ('etheroleic acid'). With lesser efficiency [1-14C]alpha-linolenic acid or [1-14C](9Z,11E, 13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid (13-HPOT) are converted in this way into (9Z,11E,1'E,1'E,3'Z)-12-(1',3'-hexadienyloxy)-9,11- dodecadienoic acid ('etherolenic acid'). Thus, garlic bulbs possess the activity of a new 13-hydroperoxide-specific divinyl ether synthase.

摘要

用大蒜鳞茎汁与[1-¹⁴C]亚油酸或[1-¹⁴C]-(9Z,11E,13S)-13-氢过氧-9,11-十八碳二烯酸（13-HPOD）一起孵育，会形成一种主要的标记产物，即新型二乙烯基醚(9Z,11E,1'E)-12-(1'-己烯氧基)-9,11-十二碳二烯酸（“醚油酸”）。效率较低的情况下，[1-¹⁴C]α-亚麻酸或1-¹⁴C-13-氢过氧-9,11,15-十八碳三烯酸（13-HPOT）会以这种方式转化为(9Z,11E,1'E,1'E,3'Z)-12-(1',3'-己二烯氧基)-9,11-十二碳二烯酸（“醚亚麻油酸”）。因此，大蒜鳞茎具有一种新的13-氢过氧化物特异性二乙烯基醚合酶的活性。