Grechkin A N, Hamberg M
Institute of Biology, Russian Academy of Sciences, Kazan, Russia.
FEBS Lett. 1996 Jun 17;388(2-3):112-4. doi: 10.1016/0014-5793(96)00536-4.
Sub-cellular localization and some properties of 13-hydroperoxide-specific divinyl ether synthase from garlic bulbs were studied. Sub-cellular fractions from garlic bulbs were incubated with 1-(14)C-13-hydroperoxy-9,11-octadecadienoic acid (13-HPOD). The predominant part of divinyl ether synthase activity from garlic bulbs was found in the microsomal fraction. The enzyme utilizes 13(S)-HPOD as its preferential substrate. Other hydroperoxides, including 9(S)-HPOD, gave much poorer yields of divinyl ethers. Unreacted hydroperoxide after incubation of 13(R,S)-HPOD with enzyme was composed of up to 94% 13(R)-HPOD. Thus, divinyl ether synthase possesses stereoselectivity, utilizing preferentially the (S)-enantiomer.
对大蒜鳞茎中13-氢过氧化物特异性二乙烯基醚合酶的亚细胞定位和一些特性进行了研究。将大蒜鳞茎的亚细胞组分与1-(14)C-13-氢过氧-9,11-十八碳二烯酸（13-HPOD）一起孵育。发现大蒜鳞茎中二乙烯基醚合酶活性的主要部分存在于微粒体组分中。该酶优先利用13(S)-HPOD作为底物。其他氢过氧化物，包括9(S)-HPOD，生成二乙烯基醚的产率要低得多。13(R,S)-HPOD与酶孵育后未反应的氢过氧化物中高达94%是13(R)-HPOD。因此，二乙烯基醚合酶具有立体选择性，优先利用(S)-对映体。
