• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

棉花 GhWIN2 基因激活表皮生物合成途径，并影响水杨酸和茉莉酸生物合成途径。

The cotton GhWIN2 gene activates the cuticle biosynthesis pathway and influences the salicylic and jasmonic acid biosynthesis pathways.

机构信息

College of Science, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China.

State Key Laboratory of Cotton Biology, Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang, 455000, China.

出版信息

BMC Plant Biol. 2019 Aug 28;19(1):379. doi: 10.1186/s12870-019-1888-6.

DOI:10.1186/s12870-019-1888-6
PMID:31455203
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6712776/
Abstract

BACKGROUND

Metabolic pathways are interconnected and yet relatively independent. Genes involved in metabolic modules are required for the modules to run. Study of the relationships between genes and metabolic modules improves the understanding of metabolic pathways in plants. The WIN transcription factor activates the cuticle biosynthesis pathway and promotes cuticle biosynthesis. The relationship between the WIN transcription factor and other metabolic pathways is unknown. Our aim was to determine the relationships between the main genes involved in cuticle biosynthesis and those involved in other metabolic pathways. We did this by cloning a cotton WIN gene, GhWIN2, and studying its influence on other pathways.

RESULTS

As with other WIN genes, GhWIN2 regulated expression of cuticle biosynthesis-related genes, and promoted cuticle formation. Silencing of GhWIN2 resulted in enhanced resistance to Verticillium dahliae, caused by increased content of salicylic acid (SA). Moreover, silencing of GhWIN2 suppressed expression of jasmonic acid (JA) biosynthesis-related genes and content. GhWIN2 positively regulated the fatty acid biosynthesis pathway upstream of the JA biosynthesis pathway. Silencing of GhWIN2 reduced the content of stearic acid, a JA biosynthesis precursor.

CONCLUSIONS

GhWIN2 not only regulated the cuticle biosynthesis pathway, but also positively influenced JA biosynthesis and negatively influenced SA biosynthesis.

摘要

背景

代谢途径是相互关联的，但又相对独立。参与代谢模块的基因是模块运行所必需的。研究基因与代谢模块之间的关系可以提高对植物代谢途径的理解。WIN 转录因子激活角质层生物合成途径并促进角质层生物合成。WIN 转录因子与其他代谢途径之间的关系尚不清楚。我们的目的是确定角质层生物合成的主要基因与其他代谢途径之间的关系。我们通过克隆棉花 WIN 基因 GhWIN2 并研究其对其他途径的影响来实现这一目标。

结果

与其他 WIN 基因一样，GhWIN2 调节角质层生物合成相关基因的表达，并促进角质层形成。GhWIN2 的沉默导致水杨酸 (SA) 含量增加，从而增强了对黄萎病菌的抗性。此外，GhWIN2 的沉默抑制了茉莉酸 (JA) 生物合成相关基因的表达和含量。GhWIN2 正向调控 JA 生物合成途径上游的脂肪酸生物合成途径。GhWIN2 的沉默降低了 JA 生物合成前体硬脂酸的含量。

结论

GhWIN2 不仅调节角质层生物合成途径，还正向影响 JA 生物合成，负向影响 SA 生物合成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/fd3de95db170/12870_2019_1888_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/6213c260f8ff/12870_2019_1888_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/6005ed759784/12870_2019_1888_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/6a5768cd5903/12870_2019_1888_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/1c6fdbf282c9/12870_2019_1888_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/fd380ed2f59a/12870_2019_1888_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/0d5d7a4d7ea5/12870_2019_1888_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/fd3de95db170/12870_2019_1888_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/6213c260f8ff/12870_2019_1888_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/6005ed759784/12870_2019_1888_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/6a5768cd5903/12870_2019_1888_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/1c6fdbf282c9/12870_2019_1888_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/fd380ed2f59a/12870_2019_1888_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/0d5d7a4d7ea5/12870_2019_1888_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f307/6712776/fd3de95db170/12870_2019_1888_Fig7_HTML.jpg

相似文献

1
The cotton GhWIN2 gene activates the cuticle biosynthesis pathway and influences the salicylic and jasmonic acid biosynthesis pathways.棉花 GhWIN2 基因激活表皮生物合成途径，并影响水杨酸和茉莉酸生物合成途径。
BMC Plant Biol. 2019 Aug 28;19(1):379. doi: 10.1186/s12870-019-1888-6.
2
Suppression of the homeobox gene HDTF1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton.抑制同源盒基因 HDTF1 可增强棉花对黄萎病菌和灰霉病菌的抗性。
J Integr Plant Biol. 2016 May;58(5):503-13. doi: 10.1111/jipb.12432. Epub 2015 Nov 21.
3
Proteomic and virus-induced gene silencing (VIGS) Analyses reveal that gossypol, brassinosteroids, and jasmonic acid contribute to the resistance of cotton to Verticillium dahliae.蛋白质组学和病毒诱导的基因沉默（VIGS）分析表明，棉酚、油菜素内酯和茉莉酸有助于棉花抵抗黄萎病。
Mol Cell Proteomics. 2013 Dec;12(12):3690-703. doi: 10.1074/mcp.M113.031013. Epub 2013 Sep 9.
4
GhCPK33 Negatively Regulates Defense against by Phosphorylating GhOPR3.GhCPK33 通过磷酸化 GhOPR3 负调控对 的防御。
Plant Physiol. 2018 Oct;178(2):876-889. doi: 10.1104/pp.18.00737. Epub 2018 Aug 27.
5
The GhEB1C gene mediates resistance of cotton to Verticillium wilt.GhEB1C 基因介导棉花对黄萎病的抗性。
Planta. 2024 Oct 1;260(5):110. doi: 10.1007/s00425-024-04524-w.
6
A Ve homologous gene from Gossypium barbadense, Gbvdr3, enhances the defense response against Verticillium dahliae.来自海岛棉的一个Ve同源基因Gbvdr3增强了对大丽轮枝菌的防御反应。
Plant Physiol Biochem. 2016 Jan;98:101-11. doi: 10.1016/j.plaphy.2015.11.015. Epub 2015 Nov 24.
7
Dynamic characteristics and functional analysis provide new insights into long non-coding RNA responsive to Verticillium dahliae infection in Gossypium hirsutum.动态特征和功能分析为长非编码 RNA 响应棉花黄萎病菌感染提供了新的见解。
BMC Plant Biol. 2021 Feb 1;21(1):68. doi: 10.1186/s12870-021-02835-8.
8
GhODO1, an R2R3-type MYB transcription factor, positively regulates cotton resistance to Verticillium dahliae via the lignin biosynthesis and jasmonic acid signaling pathway.GhODO1，一种 R2R3 型 MYB 转录因子，通过木质素生物合成和茉莉酸信号通路正向调控棉花对黄萎病的抗性。
Int J Biol Macromol. 2022 Mar 15;201:580-591. doi: 10.1016/j.ijbiomac.2022.01.120. Epub 2022 Jan 21.
9
GhMYB4 downregulates lignin biosynthesis and enhances cotton resistance to Verticillium dahliae.GhMYB4 下调木质素生物合成并增强棉花对黄萎病菌的抗性。
Plant Cell Rep. 2021 Apr;40(4):735-751. doi: 10.1007/s00299-021-02672-x. Epub 2021 Feb 27.
10
Cotton S-adenosylmethionine decarboxylase-mediated spermine biosynthesis is required for salicylic acid- and leucine-correlated signaling in the defense response to Verticillium dahliae.棉花S-腺苷甲硫氨酸脱羧酶介导的精胺生物合成是棉花对大丽轮枝菌防御反应中水杨酸和亮氨酸相关信号传导所必需的。
Planta. 2016 Apr;243(4):1023-39. doi: 10.1007/s00425-015-2463-5. Epub 2016 Jan 13.

引用本文的文献

1
Genome-wide identification and expression analysis of ACS and ACO gene family in Ziziphus jujuba mill during fruit ripening.枣果实成熟过程中ACS和ACO基因家族的全基因组鉴定与表达分析
Sci Rep. 2025 May 24;15(1):18106. doi: 10.1038/s41598-025-03014-7.
2
Shedding New Light on the Hull-Pericarp Adhesion Mechanisms of Barley Grains by Transcriptomics Analysis of Isogenic and Lines.通过对近等基因系的转录组学分析揭示大麦籽粒颖果-果皮粘连机制
Int J Mol Sci. 2024 Dec 6;25(23):13108. doi: 10.3390/ijms252313108.
3
Plant disease resistance outputs regulated by AP2/ERF transcription factor family.

本文引用的文献

1
A Cotton Cyclin-Dependent Kinase E Confers Resistance to Mediated by Jasmonate-Responsive Pathway.一种棉花细胞周期蛋白依赖性激酶E赋予对茉莉酸响应途径介导的抗性。
Front Plant Sci. 2018 May 24;9:642. doi: 10.3389/fpls.2018.00642. eCollection 2018.
2
Genome-wide identification of ABA receptor PYL family and expression analysis of in response to ABA and osmotic stress in .全基因组范围内ABA受体PYL家族的鉴定以及其在响应ABA和渗透胁迫时的表达分析
PeerJ. 2017 Dec 6;5:e4126. doi: 10.7717/peerj.4126. eCollection 2017.
3
Suppression of wheat TaCDK8/TaWIN1 interaction negatively affects germination of Blumeria graminis f.sp. tritici by interfering with very-long-chain aldehyde biosynthesis.
由AP2/ERF转录因子家族调控的植物抗病性输出。
Stress Biol. 2024 Jan 2;4(1):2. doi: 10.1007/s44154-023-00140-y.
4
Genome-Wide Identification and Expression Analysis of 1-Aminocyclopropane-1-Carboxylate Synthase () Gene Family in .拟南芥中1-氨基环丙烷-1-羧酸合成酶（）基因家族的全基因组鉴定与表达分析
Plants (Basel). 2023 Nov 29;12(23):4021. doi: 10.3390/plants12234021.
5
Candidate genes for field resistance to cassava brown streak disease revealed through the analysis of multiple data sources.通过多数据源分析揭示的木薯褐色条纹病田间抗性候选基因。
Front Plant Sci. 2023 Nov 3;14:1270963. doi: 10.3389/fpls.2023.1270963. eCollection 2023.
6
The R2R3-MYB transcription factor EVER controls the emission of petunia floral volatiles by regulating epicuticular wax biosynthesis in the petal epidermis.R2R3-MYB 转录因子 EVER 通过调控花瓣表皮的角质层生物合成来控制矮牵牛花香挥发物的释放。
Plant Cell. 2023 Dec 21;36(1):174-193. doi: 10.1093/plcell/koad251.
7
Insights to Gossypium defense response against Verticillium dahliae: the Cotton Cancer.对棉花防御黄萎病（Verticillium dahliae）反应的深入了解：棉花癌症。
Funct Integr Genomics. 2023 May 1;23(2):142. doi: 10.1007/s10142-023-01065-5.
8
WAX INDUCER 1 Regulates β-Diketone Biosynthesis by Mediating Expression of the Gene Cluster in Barley.WAX INDUCER 1 通过调控大麦中基因簇的表达来调节β-二酮的生物合成。
Int J Mol Sci. 2023 Apr 5;24(7):6762. doi: 10.3390/ijms24076762.
9
A comprehensive overview of cotton genomics, biotechnology and molecular biological studies.棉花基因组学、生物技术和分子生物学研究的全面概述。
Sci China Life Sci. 2023 Oct;66(10):2214-2256. doi: 10.1007/s11427-022-2278-0. Epub 2023 Mar 6.
10
Transcription factor positively modulates both jasmonic acid and wax biosynthesis in citrus.转录因子正向调控柑橘中茉莉酸和蜡的生物合成。
aBIOTECH. 2022 Nov 22;3(4):250-266. doi: 10.1007/s42994-022-00085-2. eCollection 2022 Dec.
抑制小麦TaCDK8/TaWIN1的相互作用会通过干扰超长链醛的生物合成对小麦白粉病菌的萌发产生负面影响。
Plant Mol Biol. 2018 Jan;96(1-2):165-178. doi: 10.1007/s11103-017-0687-4. Epub 2017 Dec 2.
4
Activation of ABA Receptors Gene Is Positively Correlated with Cotton Drought Tolerance in Transgenic .脱落酸受体基因的激活与转基因棉花的耐旱性呈正相关。
Front Plant Sci. 2017 Aug 23;8:1453. doi: 10.3389/fpls.2017.01453. eCollection 2017.
5
Arabidopsis ketoacyl-CoA synthase 16 (KCS16) forms C /C acyl precursors for leaf trichome and pavement surface wax.拟南芥酮酰基辅酶 A 合酶 16（KCS16）为叶片毛状体和表皮蜡质形成 C/C 酰基前体。
Plant Cell Environ. 2017 Sep;40(9):1761-1776. doi: 10.1111/pce.12981. Epub 2017 Jul 21.
6
Molecular evidence for the involvement of a polygalacturonase-inhibiting protein, GhPGIP1, in enhanced resistance to Verticillium and Fusarium wilts in cotton.棉花中多聚半乳糖醛酸酶抑制蛋白 GhPGIP1 参与增强抗黄萎病和枯萎病的分子证据。
Sci Rep. 2017 Jan 12;7:39840. doi: 10.1038/srep39840.
7
COP1 is required for UV-B-induced nuclear accumulation of the UVR8 photoreceptor.UV-B诱导的UVR8光感受器核积累需要COP1。
Proc Natl Acad Sci U S A. 2016 Jul 26;113(30):E4415-22. doi: 10.1073/pnas.1607074113. Epub 2016 Jul 12.
8
Dissecting Abscisic Acid Signaling Pathways Involved in Cuticle Formation.解析参与角质层形成的脱落酸信号通路。
Mol Plant. 2016 Jun 6;9(6):926-38. doi: 10.1016/j.molp.2016.04.001. Epub 2016 Apr 7.
9
Cotton S-adenosylmethionine decarboxylase-mediated spermine biosynthesis is required for salicylic acid- and leucine-correlated signaling in the defense response to Verticillium dahliae.棉花S-腺苷甲硫氨酸脱羧酶介导的精胺生物合成是棉花对大丽轮枝菌防御反应中水杨酸和亮氨酸相关信号传导所必需的。
Planta. 2016 Apr;243(4):1023-39. doi: 10.1007/s00425-015-2463-5. Epub 2016 Jan 13.
10
Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae.棉花多胺氧化酶是防御Verticillium dahliae 反应中腐胺和独脚金内酯信号传导所必需的。
Plant J. 2015 Sep;83(6):962-75. doi: 10.1111/tpj.12941. Epub 2015 Aug 26.