Atawodi S E, Mende P, Pfundstein B, Preussmann R, Spiegelhalder B
Department of Environmental Carcinogens/0325, German Cancer Research Centre, Heidelberg.
Food Chem Toxicol. 1995 Aug;33(8):625-30. doi: 10.1016/0278-6915(95)00035-z.
Three varieties of kola nut, Cola acuminata, C. nitida and Garcinia cola, of Nigerian origin, were analysed for their content of primary and secondary amines, and assessed for their relative methylating potential due to nitrosamide formation. Primary and secondary amines were determined as benzene sulfonamides by gas chromatography/thermal energy analysis (GC/TEA). Dimethylamine, methylamine, ethylamine and isopentylamine were detected in all kola nut varieties, while pyrrolidine, piperidine and isobutylamine were detected in one or more varieties. Estimated average total daily intake of aliphatic amines by a typical kola nut chewer varied from 260 to 1040 micrograms/day for secondary amines and from 2430 to 9710 micrograms/day for primary amines. Methylating activity of the nitrosated kola nuts, expressed as N-nitroso-N-methylurea equivalents, was also determined by GC/TEA. Methylating activity was significantly higher in kola nuts (170-490 micrograms/kg) than has ever been reported for a fresh plant product. These data suggest that the possible role of kola nut chewing in human cancer aetiology should be explored in countries where kola nuts are widely consumed as stimulants.
对原产于尼日利亚的三种可乐果品种，即锐尖可乐果、光亮可乐果和可乐青果，进行了伯胺和仲胺含量分析，并评估了它们因亚硝胺形成而产生的相对甲基化潜力。通过气相色谱/热能分析（GC/TEA）将伯胺和仲胺测定为苯磺酰胺。在所有可乐果品种中均检测到二甲胺、甲胺、乙胺和异戊胺，而在一个或多个品种中检测到吡咯烷、哌啶和异丁胺。典型的可乐果咀嚼者估计的脂肪胺平均每日总摄入量，仲胺为260至1040微克/天，伯胺为2430至9710微克/天。经亚硝化的可乐果的甲基化活性，以N-亚硝基-N-甲基脲当量表示，也通过GC/TEA测定。可乐果中的甲基化活性（170 - 490微克/千克）显著高于以往报道的新鲜植物产品。这些数据表明，在可乐果作为兴奋剂被广泛食用的国家，应探讨咀嚼可乐果在人类癌症病因学中可能发挥的作用。
