Atawodi S E, Spiegelhalder B
Department of Environmental Carcinogens, German Cancer Research Center, Heidelberg.
Cancer Lett. 1994 Apr 29;79(1):107-15. doi: 10.1016/0304-3835(94)90070-1.
Twenty-seven tropical plants of medicinal importance were analysed for primary and secondary amines by chemiluminescence detection on a Thermal Energy Analyzer (TEA) modified for use on 'nitrogen mode' following derivatization with benzene sulphonyl chloride (BSC) and gas chromatographic (GC) separation of their sulphonamides. Nitrite was determined by colorimetry at 540 nm after diazotization with sulphanilamide and coupling with N-(1-naphthyl)ethylenediamine to form an azo dye. Nitrate was determined as nitrite following on-line reduction by granulated cadmium. Dimethylamine in the range of 0.5 ppm to 18.2 ppm was detected in 96% of samples, while pyrrolidine ranged between 0.7 ppm and 12.78 ppm in 14 samples. Isobutylamine, methylamine and ethylamine were the most ubiquitous primary amines. Largest number of secondary amines (four) was found in Azadirachta indica (Neem) while largest number of primary amines (six) was detected in Azadirachta indica and Tamarindus indica (Tsamiya) which also contained the highest amount of total primary amines (148.8 ppm). Nitrate and nitrite were seldom found in plant extracts whose pH were generally below 7.0. These findings suggests that early exposures to precursors of N-nitroso compounds via medicinal plants might contribute to total risk posed by environmental carcinogens in Nigeria.
对27种具有药用价值的热带植物进行了分析，采用热能量分析仪（TEA）通过化学发光检测法测定其伯胺和仲胺。该热能量分析仪经改装后用于“氮模式”，先用苯磺酰氯（BSC）进行衍生化处理，然后对其磺酰胺进行气相色谱（GC）分离。亚硝酸盐通过与磺胺进行重氮化反应并与N-（1-萘基）乙二胺偶联形成偶氮染料后，在540nm处用比色法测定。硝酸盐通过颗粒状镉在线还原后测定为亚硝酸盐。在96%的样品中检测到二甲胺含量在0.5ppm至18.2ppm之间，而在14个样品中吡咯烷含量在0.7ppm至12.78ppm之间。异丁胺、甲胺和乙胺是最普遍存在的伯胺。在印楝（Neem）中发现的仲胺数量最多（四种），而在印楝和罗望子（Tsamiya）中检测到的伯胺数量最多（六种），这两种植物中总伯胺含量也最高（148.8ppm）。在pH值通常低于7.0的植物提取物中很少发现硝酸盐和亚硝酸盐。这些发现表明，通过药用植物早期接触N-亚硝基化合物的前体可能会增加尼日利亚环境致癌物带来的总体风险。
