Morrison C E, Lichstein H C
J Bacteriol. 1976 Mar;125(3):864-71. doi: 10.1128/jb.125.3.864-871.1976.
A steady-state level of about 240 nmol/mg (dry wt) occurs during lysine transport in Saccharomyces cerevisiae. No subsequent efflux of the accumulated amino acid was detected. Two transport systems mediate lysine transport, a high-affinity, lysine-specific system and an arginine-lysine system for which lysine exhibits a lower affinity. Preloading with lysine, arginine, glutamic acid, or aspartic acid inhibited lysine transport activity; preloading with glutamine, glycine, methionine, phenylalanine, or valine had little effect; however, preloading with histidine stimulated lysine transport activity. These preloading effects correlated with fluctuations in the intracellular lysine and/or arginine pool: lysine transport activity was inhibited when increases in the lysine and/or arginine pool occurred and was stimulated when decreases in the lysine and/or arginine pool occurred. After addition of lysine to a growing culture, lysine transport activity was inhibited more than threefold in one-third of the doubling time of the culture. These results indicate that the lysine-specific and arginine-lysine transport systems are regulated by feedback inhibition that may be mediated by intracellular lysine and arginine.
在酿酒酵母的赖氨酸转运过程中，会出现约240纳摩尔/毫克（干重）的稳态水平。未检测到积累的氨基酸随后有外流情况。两种转运系统介导赖氨酸转运，一种是高亲和力、赖氨酸特异性系统，另一种是赖氨酸亲和力较低的精氨酸 - 赖氨酸系统。用赖氨酸、精氨酸、谷氨酸或天冬氨酸预加载会抑制赖氨酸转运活性；用谷氨酰胺、甘氨酸、蛋氨酸、苯丙氨酸或缬氨酸预加载影响不大；然而，用组氨酸预加载会刺激赖氨酸转运活性。这些预加载效应与细胞内赖氨酸和/或精氨酸池的波动相关：当赖氨酸和/或精氨酸池增加时，赖氨酸转运活性受到抑制，而当赖氨酸和/或精氨酸池减少时，转运活性受到刺激。向正在生长的培养物中添加赖氨酸后，在培养物倍增时间的三分之一内，赖氨酸转运活性被抑制了三倍以上。这些结果表明，赖氨酸特异性和精氨酸 - 赖氨酸转运系统受反馈抑制调节，这种抑制可能由细胞内的赖氨酸和精氨酸介导。
