Pedrozo H A, Schwartz Z, Nakaya H, Harrison J L, Dean D D, Wiederhold M L, Boyan B D
Department of Physiology, University of Texas Health Sciences Center at San Antonio 78284-7756, USA.
J Comp Physiol A. 1995 Oct;177(4):415-25. doi: 10.1007/BF00187478.
A novel organ culture system has been developed to study the regulation of statoconia production in the gravity sensing organ in Aplysia californica. Statocysts were cultured in Leibovitz (L15) medium supplemented with salts and Aplysia haemolymph for four days at 17 degrees C. The viability of the system was evaluated by examining four parameters: statocyst morphology, the activity of the mechanosensory cilia in the statocyst, production of new statoconia during culture and change in statoconia volume after culture. There were no morphological differences in statocysts before and after culture when ciliary beating was maintained. There was a 29% increase in the number of statoconia after four days in culture. Mean statocyst, statolith and statoconia volumes were not affected by culture conditions. The presence of carbonic anhydrase in the statocysts was shown using immunohistochemistry. When statocysts were cultured in the presence of 4.0 x 10(-4) M acetazolamide to inhibit the enzyme activity, there was a decrease in statoconia production and statoconia volume, indicating a role for this enzyme in statoconia homeostasis, potentially via pH regulation. These studies are the first to report a novel system for the culture of statocysts and show that carbonic anhydrase is involved in the regulation of statoconia volume and production.
已开发出一种新型器官培养系统，用于研究加州海兔重力感应器官中平衡石产生的调节机制。平衡囊在补充了盐分和海兔血淋巴的莱博维茨（L15）培养基中于17摄氏度培养四天。通过检查四个参数来评估该系统的活力：平衡囊形态、平衡囊内机械感觉纤毛的活性、培养期间新平衡石的产生以及培养后平衡石体积的变化。当纤毛跳动得以维持时，培养前后平衡囊在形态上没有差异。培养四天后，平衡石的数量增加了29%。平均平衡囊、平衡石和平衡石体积不受培养条件的影响。使用免疫组织化学方法显示了平衡囊中碳酸酐酶的存在。当在4.0×10⁻⁴ M乙酰唑胺存在的情况下培养平衡囊以抑制该酶的活性时，平衡石的产生和平衡石体积均有所下降，这表明该酶可能通过调节pH值在平衡石稳态中发挥作用。这些研究首次报道了一种用于培养平衡囊的新型系统，并表明碳酸酐酶参与了平衡石体积和产生的调节。
