Kawano S, Tsukamoto T, Ohtaguro H, Tsutsumi H, Takahashi T, Miura I, Mukoyama R, Aboshi H, Komuro T
Department of Legal Medicine, Nihon University School of Dentistry, Tokyo, Japan.
Nihon Hoigaku Zasshi. 1995 Jun;49(3):193-8.
We studied sex determination from dental calculus by the PCR method using primers which recognize DYZ3 region of Y-chromosome and DXZ1 of X-chromosome. The amounts of DNA obtained from 1.7-25.0 mg of dental calculus samples ranged 0.6-26.4 micrograms, and the majority of DNA was low molecular weight DNA. After PCR amplification, Y and X products were observed by the method of polyacrylamide gel electrophoresis. Both Y and X amplification products were observed in 26 out of 29 male samples, while, Y products was not detected in two samples and neither Y nor X product was detected in one sample. In 17 out of 20 female samples, only X product was observed, while, Y and X products were observed in three samples. The minimum amount of DNA for sex determination was 3 pg. Sex determination using DNA in dental calculus will be quite useful for forensic application because it can be done without destruction of morphological characteristics of teeth.
我们使用识别Y染色体DYZ3区域和X染色体DXZ1的引物，通过PCR方法研究了牙结石中的性别鉴定。从1.7 - 25.0毫克牙结石样本中获得的DNA量在0.6 - 26.4微克之间，并且大多数DNA是低分子量DNA。PCR扩增后，通过聚丙烯酰胺凝胶电泳法观察Y和X产物。29个男性样本中有26个同时观察到Y和X扩增产物，而有2个样本未检测到Y产物，1个样本既未检测到Y产物也未检测到X产物。20个女性样本中有
