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日本人群中的三个短串联重复序列位点

Three short tandem repeat loci in Japanese population.

作者信息

Fujita H, Naito E, Dewa K, Yamanouchi H

机构信息

Department of Legal Medicine, Niigata University School of Medicine, Japan.

出版信息

Nihon Hoigaku Zasshi. 1995 Jun;49(3):213-21.

PMID:7674562
Abstract

Allele frequencies of three short tandem repeat loci, vWF, TH and GCG, were examined for 210 unrelated Japanese individuals. Eight alleles were identified with PCR and gel electrophoresis for vWF, 7 for TH and 13 for GCG, indicating that these loci are highly polymorphic. Combined discrimination power of the 3 STRs was 0.995. These results imply that the loci are useful as probes for the identification of Japanese individuals. Thus, we applied the probes to the identification of a forensic specimen that was decomposed severely. The vWF and TH probes successfully gave clear bands in gel electrophoresis, but GCG failed to be typed because of a lot of shadow bands. The results suggests the usefulness of at least the former two probes in forensic studies.

摘要

对210名无亲缘关系的日本人检测了3个短串联重复序列位点(vWF、TH和GCG)的等位基因频率。通过PCR和凝胶电泳,vWF鉴定出8个等位基因,TH鉴定出7个,GCG鉴定出13个,表明这些位点具有高度多态性。3个STR的联合鉴别力为0.995。这些结果表明这些位点可作为日本人个体识别的探针。因此,我们将这些探针应用于一个严重腐败的法医样本的鉴定。vWF和TH探针在凝胶电泳中成功给出清晰条带,但GCG因大量拖带而未能分型。结果表明至少前两个探针在法医研究中有用。

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