Fujita H, Naito E, Dewa K, Yamanouchi H
Department of Legal Medicine, Niigata University School of Medicine, Japan.
Nihon Hoigaku Zasshi. 1995 Jun;49(3):213-21.
Allele frequencies of three short tandem repeat loci, vWF, TH and GCG, were examined for 210 unrelated Japanese individuals. Eight alleles were identified with PCR and gel electrophoresis for vWF, 7 for TH and 13 for GCG, indicating that these loci are highly polymorphic. Combined discrimination power of the 3 STRs was 0.995. These results imply that the loci are useful as probes for the identification of Japanese individuals. Thus, we applied the probes to the identification of a forensic specimen that was decomposed severely. The vWF and TH probes successfully gave clear bands in gel electrophoresis, but GCG failed to be typed because of a lot of shadow bands. The results suggests the usefulness of at least the former two probes in forensic studies.
对210名无亲缘关系的日本人检测了3个短串联重复序列位点(vWF、TH和GCG)的等位基因频率。通过PCR和凝胶电泳,vWF鉴定出8个等位基因,TH鉴定出7个,GCG鉴定出13个,表明这些位点具有高度多态性。3个STR的联合鉴别力为0.995。这些结果表明这些位点可作为日本人个体识别的探针。因此,我们将这些探针应用于一个严重腐败的法医样本的鉴定。vWF和TH探针在凝胶电泳中成功给出清晰条带,但GCG因大量拖带而未能分型。结果表明至少前两个探针在法医研究中有用。