Miyazaki S, Imaizumi M, Machida H
Biology Laboratory, Research & Development Division, Yamasa Corporation, Chiba, Japan.
Methods Find Exp Clin Pharmacol. 1995 Mar;17(2):121-7.
We examined the effects of anxiolytics and anxiogenics on evaluation of learning and memory in the elevated plus-maze test in mice. Transfer latency (TL), the time mice took to move from the open arm to the enclosed arm, was used as an index of learning and memory. The TL on day 2 was shorter than that on day 1 with the maze at a height of 40 cm, and was not affected by anxiolytics and anxiogenics such as 8-OH-DPAT (0.4 mg/kg), picrotoxin (2 mg/kg), and FG-7142 (20 mg/kg), but was affected by diazepam (8 mg/kg). On the other hand, the TL on day 2 was prolonged by changing the experimental room between day 1 and day 2. TL on day 1 and day 2. TL on day 2 was prolonged by treatment with scopolamine before the trial on day 1 compared with vehicle-treated groups. This scopolamine-induced prolongation of TL was reversed by pretreatment with physostigmine and piracetam. The TL on day 2 was also prolonged by treatment with cycloheximide or normobaric hypoxia initiated immediately after the trial on day 1. The cycloheximide- and hypoxia-induced prolongation of TL were reversed by pretreatment with piracetam. These results indicate that disruption of learning and memory and its amelioration, induced by the treatments described above, can be detected using the plus-maze test, suggesting that the elevated plus-maze test could be used for evaluation of learning and memory without influence of anxiolytics and anxiogenics.
我们在小鼠高架十字迷宫试验中研究了抗焦虑药和致焦虑药对学习和记忆评估的影响。转移潜伏期（TL），即小鼠从开放臂移动到封闭臂所需的时间，被用作学习和记忆的指标。当迷宫高度为40厘米时，第2天的TL比第1天短，且不受8-OH-DPAT（0.4毫克/千克）、印防己毒素（2毫克/千克）和FG-7142（20毫克/千克）等抗焦虑药和致焦虑药的影响，但受地西泮（8毫克/千克）影响。另一方面，通过在第1天和第2天之间更换实验室，第2天的TL延长。与溶剂处理组相比，在第1天试验前用东莨菪碱处理可延长第2天的TL。东莨菪碱诱导的TL延长可被毒扁豆碱和吡拉西坦预处理逆转。在第1天试验后立即用环己酰亚胺或常压缺氧处理也可延长第2天的TL。环己酰亚胺和缺氧诱导的TL延长可被吡拉西坦预处理逆转。这些结果表明，使用十字迷宫试验可以检测到上述处理诱导的学习和记忆破坏及其改善情况，提示高架十字迷宫试验可用于评估学习和记忆，而不受抗焦虑药和致焦虑药的影响。
