Sann H, Jancsó G, Ambrus A, Pierau F K
MPI Für physiologische und klinische Forschung, W. G. Kerckhoff-Institut, Nauheim, Germany.
Neuroscience. 1995 Aug;67(4):953-66. doi: 10.1016/0306-4522(95)00102-o.
Quantitative immunohistochemistry was used to study the innervation of the ureter in adult rats pretreated with capsaicin as neonates (50 mg/kg) or as adults (100-150 mg/kg, 10-22 days prior to being killed) using antibodies against protein gene-product 9.5, neuron-specific enolase, substance P, calcitonin gene-related peptide, neuropeptide Y, dopamine-beta-hydroxylase and vasoactive intestinal polypeptide. The number of calcitonin gene-related peptide- and substance P-containing fibres was reduced in the subepithelial plexus (adult capsaicin treatment < 1%, neonatal treatment < 5% of control), the submucosa (adult treatment < 11%; neonatal treatment < 51%) and in the smooth muscle layer and adventitia (adult treatment < 11%; neonatal treatment < 58%). Fibres immunoreactive for protein gene-product 9.5, a general neuronal marker, were reduced to 11% (adult treatment) or 0.5% (neonatal treatment) in the subepithelial plexus, but unchanged in the other layers, indicating a selective regional degeneration. In the smooth muscle layer the number of neuropeptide Y- and vasoactive intestinal polypeptide-containing nerve fibres was not altered by capsaicin. The number of neuropeptide Y fibres in the subepithelial plexus, however, was significantly increased after adult treatment (174% of control). After neonatal capsaicin treatment the intensity of the neuropeptide Y immunoreactivity was increased, more neuropeptide Y-positive nerve bundles were found and immunoreactive cell bodies were observed regularly in the adventitia of the ureter. The data indicate that capsaicin produces a selective degeneration of most afferent fibres in the subepithelial plexus of the rat ureter. This loss of capsaicin-sensitive afferent nerves evokes neuroplastic changes resulting in a hyperinnervation by neuropeptide Y-immunoreactive, presumably sympathetic fibres. The results suggest a mutual regulation of the pattern and density of innervation of peripheral target tissues by sensory and sympathetic neurons.
采用定量免疫组织化学方法，使用抗蛋白基因产物9.5、神经元特异性烯醇化酶、P物质、降钙素基因相关肽、神经肽Y、多巴胺β-羟化酶和血管活性肠肽的抗体，研究新生期（50mg/kg）或成年期（100 - 150mg/kg，处死前10 - 22天）用辣椒素预处理的成年大鼠输尿管的神经支配情况。降钙素基因相关肽和含P物质的纤维数量在大鼠输尿管上皮下丛（成年期辣椒素处理组<对照组的1%，新生期处理组<对照组的5%）、黏膜下层（成年期处理组<11%；新生期处理组<51%）以及平滑肌层和外膜（成年期处理组<11%；新生期处理组<58%）均减少。对一种通用神经元标志物蛋白基因产物9.5呈免疫反应的纤维，在上皮下丛中减少至11%（成年期处理组）或0.5%（新生期处理组），但在其他层未改变，表明存在选择性区域变性。在平滑肌层，含神经肽Y和血管活性肠肽的神经纤维数量未因辣椒素而改变。然而，成年期处理后，上皮下丛中神经肽Y纤维数量显著增加（为对照组的174%）。新生期辣椒素处理后，神经肽Y免疫反应强度增加，发现更多神经肽Y阳性神经束，并且在输尿管外膜中经常观察到免疫反应性细胞体。数据表明，辣椒素使大鼠输尿管上皮下丛中的大多数传入纤维发生选择性变性。这种对辣椒素敏感的传入神经的丧失引发神经可塑性变化，导致由神经肽Y免疫反应性（可能是交感神经纤维）引起的神经支配过度。结果提示感觉神经元和交感神经元对外周靶组织神经支配模式和密度存在相互调节作用。
