Weiger R, Netuschil L, von Ohle C, Schlagenhauf U, Brecx M
Department of Conservative Dentistry, School of Dental Medicine, University of Tübingen, Germany.
Oral Microbiol Immunol. 1995 Apr;10(2):93-7. doi: 10.1111/j.1399-302x.1995.tb00125.x.
The objective of this investigation was to determine the microbial generation time during early human supragingival plaque formation. On 2 occasions, 13 participants refrained from all oral hygiene measures for periods of 1, 2, 4, 8, 24 and 72 h. Plaque was sampled from defined areas of the teeth 13, 14, 15, 23, 24 and 25. Two independent methods were used to estimate the total number of viable cells: colony-forming units BCVF, total bacterial counts (BC) as enumerated by microscopic examination, and the percentage of vital bacteria using a vital fluorescence technique (VF) were recorded. The total number of vital microorganisms was calculated by BCVF (= BC x VF). The generation time based on either colony-forming units or BCVF increased significantly with plaque age. During the first interval (1-4 h), the microbial multiplication took slightly less than 1 h. After the establishment of a more mature plaque (24-72 h), the replicating time was between 12.5 h (BCVF) and 14.8 h (colony-forming units). It was concluded that the microbial generation time recorded at the initial stages of plaque formation is considerably lower than that found in older plaque.
本研究的目的是确定人类早期龈上菌斑形成过程中的微生物代时。在两个不同时间段，13名参与者分别在1、2、4、8、24和72小时内停止所有口腔卫生措施。从牙齿13、14、15、23、24和25的特定区域采集菌斑。使用两种独立的方法估计活细胞总数：菌落形成单位（BCVF）、通过显微镜检查计数的总细菌数（BC），并记录使用活荧光技术（VF）检测的活细菌百分比。活微生物总数通过BCVF（=BC×VF）计算得出。基于菌落形成单位或BCVF的代时均随菌斑年龄显著增加。在第一个时间段（1 - 4小时），微生物繁殖耗时略少于1小时。在形成更成熟的菌斑后（24 - 72小时），复制时间在12.5小时（BCVF）至14.8小时（菌落形成单位）之间。得出的结论是，在菌斑形成初期记录的微生物代时显著低于在较老菌斑中发现的代时。
