Littlewood D T, Johnston D A
Department of Biology, King's College London.
Parasitology. 1995 Aug;111 ( Pt 2):167-75. doi: 10.1017/s003118200006491x.
Partial 28S ribosomal RNA (rRNA) gene sequences, including the variable domains D1, D2 and D3, were determined for representative species from the 4 Schistosoma species groups. On an alignment of 1345 bp from S. mansoni, S. haematobium, S. spindale and S. japonicum (with Heterobilharzia americana chosen as an outgroup), both maximum likelihood and maximum parsimony analyses provide a robust molecular phylogeny for the genus; ((((S. haematobium, S. spindale), S. mansoni), S. japonicum), H. americana). When analysed separately, both domain D1 and domain D2 yielded similarly informative data whereas D3 failed to resolve the phylogeny. These results confirm a phylogeny previously suggested by 18S rRNA gene sequences, corroborating the status of S. spindale as a sister taxon to S. haematobium, and demonstrate the utility of 28S rRNA gene sequence data for resolving phylogenies within the Schistosomatidae.
测定了来自4个血吸虫物种组代表性物种的部分28S核糖体RNA（rRNA）基因序列，包括可变区D1、D2和D3。对曼氏血吸虫、埃及血吸虫、间插血吸虫和日本血吸虫（以美洲异双盘吸虫作为外类群）的1345 bp序列进行比对后，最大似然法和最大简约法分析均为该属提供了可靠的分子系统发育树；（（（埃及血吸虫，间插血吸虫），曼氏血吸虫），日本血吸虫），美洲异双盘吸虫）。单独分析时，D1区和D2区产生了类似的信息数据，而D3区未能解析系统发育关系。这些结果证实了先前由18S rRNA基因序列提出的系统发育关系，支持了间插血吸虫作为埃及血吸虫姐妹分类单元的地位，并证明了28S rRNA基因序列数据在解析裂体科内系统发育关系方面的实用性。
