Berger R
Unité INSERM U 301, Institut de Génétique Moléculaire, Paris, France.
Pathol Biol (Paris). 1995 Mar;43(3):175-80.
Fluorescence in situ hybridization (FISH) techniques has been considerably developed over the past years. They have brought new important data when applied to hematological malignancies: studies of aneuploidies on interphase nuclei at diagnosis and in the follow-up of the patients, combination with immunophenotype studies allowing to determine the lineage of the cells studied, simultaneous use of several molecular probes by multicolor hybridization, detection of chromosome rearrangements difficult to ascertain with usual techniques, delineation of the breakpoints in chromosome abnormalities. Examples of these applications are presented and discussed.
荧光原位杂交（FISH）技术在过去几年中有了长足的发展。当应用于血液系统恶性肿瘤时，它们带来了新的重要数据：在诊断时和患者随访中对间期核的非整倍体研究，与免疫表型研究相结合以确定所研究细胞的谱系，通过多色杂交同时使用几种分子探针，检测用常规技术难以确定的染色体重排，描绘染色体异常中的断点。本文展示并讨论了这些应用的实例。
