Nam M J, Thore C, Busija D
Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1083, USA.
Prostaglandins Leukot Essent Fatty Acids. 1995 Jul;53(1):69-72. doi: 10.1016/0952-3278(95)90086-1.
Experiments were done to determine whether interleukin 1 alpha (IL-1 alpha) induces prostaglandin (PG) synthesis in cultured astroglia and to examine the role of enhanced cyclooxygenase 2 (COX 2) synthesis in these responses. Immunologically identified fetal ovine astrocytes were exposed to IL-1 alpha or to media alone. Application of 10 ng/ml IL-1 alpha increased the production of PGF2 alpha for 2-24 h. Coincubation of IL-1 alpha with actinomycin D (1 microgram/ml) or cycloheximide (10 micrograms/ml) completely blocked the increase in PGF2 alpha. Immunoblot analyses revealed that the synthesis of COX2 was not increased by IL-1 alpha at 4 h. However, with incubation of IL-1 alpha for 8-12 h, COX2 levels were increased. These results indicate that longer periods than 4 h are necessary to increase COX2 protein, suggesting that proteins other than COX2 are involved in early IL-1 alpha-induced PG synthesis.
进行实验以确定白细胞介素1α（IL-1α）是否能在培养的星形胶质细胞中诱导前列腺素（PG）合成，并研究环氧合酶2（COX 2）合成增强在这些反应中的作用。将经免疫学鉴定的胎羊星形胶质细胞暴露于IL-1α或仅暴露于培养基中。应用10 ng/ml的IL-1α可使前列腺素F2α（PGF2α）的产量在2至24小时内增加。将IL-1α与放线菌素D（1微克/毫升）或环己酰亚胺（10微克/毫升）共同孵育可完全阻断PGF2α的增加。免疫印迹分析显示，4小时时IL-1α并未增加COX2的合成。然而，在IL-1α孵育8至12小时后，COX2水平升高。这些结果表明，需要超过4小时的更长时间才能增加COX2蛋白，这表明除COX2外的其他蛋白质参与了早期IL-1α诱导的PG合成。
