Knott I, Dieu M, Burton M, Houbion A, Remacle J, Raes M
Laboratoire de Biochimie Cellulaire, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
J Rheumatol. 1994 Mar;21(3):462-6.
To investigate the regulation of the prostaglandin (PG) synthesis by interleukin 1 (IL-1) in human synovial cells and chondrocytes.
Both cell types stimulated by human recombinant IL-1 synthesized PGE2, PGF2 alpha and 6-keto-PGF1 alpha.
PGE2 was the major PG synthesized. When arachidonic acid was added exogenously at the end of the stimulation, an increase in the prostaglandin synthesis was observed after 6 and 24 h suggesting that cyclooxygenase is the limiting enzyme. Using actinomycin D and cycloheximide, PG synthesis was shown to be protein synthesis dependent. Inhibition of the constitutive cyclooxygenase by aspirin before the IL-1 stimulation confirmed that the increased prostaglandin synthesis was due to a de novo synthesis of cyclooxygenase.
This enzyme induction by IL-1 was found to be similar in both cell types.
研究白细胞介素1（IL-1）对人滑膜细胞和软骨细胞中前列腺素（PG）合成的调节作用。
两种细胞类型经人重组IL-1刺激后均合成前列腺素E2（PGE2）、前列腺素F2α（PGF2α）和6-酮-前列腺素F1α（6-keto-PGF1α）。
PGE2是合成的主要前列腺素。刺激结束时外源添加花生四烯酸后，6小时和24小时后观察到前列腺素合成增加，提示环氧化酶是限速酶。使用放线菌素D和放线菌酮，结果表明PG合成依赖于蛋白质合成。在IL-1刺激前用阿司匹林抑制组成性环氧化酶，证实前列腺素合成增加是由于环氧化酶的从头合成。
发现IL-1对这两种细胞类型的这种酶诱导作用相似。
