Post E J, Wood A K, Page M, Jeffery H E
Department of Biological Sciences, University of Sydney, NSW, Australia.
Sleep. 1995 Jun;18(5):309-16.
A healthy neonatal piglet model was developed to investigate the effects of simulated gastro-esophageal reflux (GER) on airway protective mechanisms in different sleep states. Piglets were chosen for the model because there are similarities in esophageal morphology, development of the cardiorespiratory system and sleep-wakefulness cycle between the piglet and the human infant. Unanesthetized piglets were instrumented and trained to sleep in a radiolucent, temperature-controlled box. Physiologic recordings of sleep (electroencephalogram, 'ear-o-gram', behavioral observations), cardiorespiratory function (end-tidal CO2, O2 saturation, heart rate, respiratory movements), swallowing (pharyngeal or esophageal pressures) and GER (signaled by a fall in esophageal pH) were displayed and stored on a computer. An image intensifier was used for radiographic observations. The outputs from the computer and image intensifier were synchronized and recorded on videotape. The method enabled simultaneous physiologic and radiographic observations to be made during the simulation of GER by the injection of normal NaCl, distilled water or HCl (pH 2 and 3) into either the pharynx or different sites in the esophagus. The piglet model appears to accurately reflect the normal physiologic responses of the healthy sleeping neonate.
建立了一个健康的新生仔猪模型，以研究模拟胃食管反流（GER）对不同睡眠状态下气道保护机制的影响。选择仔猪作为模型是因为仔猪与人类婴儿在食管形态、心肺系统发育以及睡眠-觉醒周期方面存在相似之处。未麻醉的仔猪被植入仪器并训练在一个可透射线、温度可控的箱子里睡觉。睡眠（脑电图、耳声图、行为观察）、心肺功能（呼气末二氧化碳、血氧饱和度、心率、呼吸运动）、吞咽（咽部或食管压力）和GER（由食管pH值下降表示）的生理记录被显示并存储在计算机上。使用影像增强器进行放射学观察。计算机和影像增强器的输出进行同步并记录在录像带上。该方法能够在通过向咽部或食管的不同部位注射正常氯化钠、蒸馏水或盐酸（pH值为2和3）来模拟GER的过程中同时进行生理和放射学观察。仔猪模型似乎能准确反映健康睡眠新生儿的正常生理反应。
