Tanji N, Yokoyama M, Takeuchi M, Terada N, Cunha G R
Department of Urology, Ehime University, Medical School, Japan.
Urol Res. 1995;23(2):127-33. doi: 10.1007/BF00307943.
The effects of suramin on the growth of seminal vesicles (SVs) of neonatal mice were investigated in vitro. SVs from 0-day-old male mice were cultured in serum-free chemically defined medium supplemented with 5 alpha-dihydrotestosterone (DHT, 10(-8) M) and insulin (10 micrograms/ml), alone and in combination. Prior to culture, SVs from 0-day-old mice had no epithelial branches. SVs cultured in medium with DHT formed numerous epithelial branches, while epithelial branching did not occur in SVs cultured without DHT. The addition of suramin (0.2 mM) to medium containing DHT inhibited the formation of epithelial branches almost completely. Removal of suramin from the medium on days 2, 4, and 6 of culture initiated the formation of epithelial branches. Suramin (0.2 mM) reversibly decreased 3H-thymidine-labeling indices (3H-LI) of both epithelium and mesenchyme of SVs cultured in medium with DHT plus insulin or DHT alone during 8 days of culture. Suramin also decreased 3H-LI of both epithelium and mesenchyme of SVs cultured in medium with insulin alone. The present study indicates that suramin reversibly inhibits not only androgen-dependent but also androgen-independent growth and ductal branching morphogenesis of neonatal mouse SVs.
在体外研究了苏拉明对新生小鼠精囊（SVs）生长的影响。将0日龄雄性小鼠的精囊在添加有5α-双氢睾酮（DHT，10⁻⁸M）和胰岛素（10微克/毫升）的无血清化学限定培养基中单独或联合培养。在培养前，0日龄小鼠的精囊没有上皮分支。在含有DHT的培养基中培养的精囊形成了许多上皮分支，而在没有DHT的培养基中培养的精囊则没有发生上皮分支。向含有DHT的培养基中添加苏拉明（0.2 mM）几乎完全抑制了上皮分支的形成。在培养的第2、4和6天从培养基中去除苏拉明会引发上皮分支的形成。在8天的培养期间，苏拉明（0.2 mM）可逆地降低了在含有DHT加胰岛素或仅含DHT的培养基中培养的精囊上皮和间充质的³H-胸腺嘧啶标记指数（³H-LI）。苏拉明还降低了仅在含有胰岛素的培养基中培养的精囊上皮和间充质的³H-LI。本研究表明，苏拉明不仅可逆地抑制新生小鼠精囊的雄激素依赖性生长，还抑制雄激素非依赖性生长以及导管分支形态发生。
