Lau S C, Scholtissek C
Institut für Virologie, Universität Giessen, Germany.
Virology. 1995 Sep 10;212(1):225-31. doi: 10.1006/viro.1995.1473.
We have discovered a new type of abortive replication in Vero cells infected with fowl plague virus. In these cells there is an enhanced splicing of the colinear mRNAs of segment 7 and presumably also of segment 8, leading to an extreme overproduction of M2 and NS2 proteins. The cleavage of the hemagglutinin (HA) into HA1 and HA2 and the processing of its carbohydrate side chains are markedly retarded and incomplete. Although some of the HA is incorporated into the plasma membrane, leading to a positive hemadsorption, most of it accumulates in a discrete compartment close to the nuclear membrane, representing presumably the reticuloendothel and/or the Golgi network. Neuraminidase activity in Vero cells is extremely low. The nucleoprotein is normally released from nuclei late in infection. Very little infectious virus is released, and its spread is highly impeded.
我们在感染了禽痘病毒的Vero细胞中发现了一种新型的流产性复制。在这些细胞中，第7节段共线性mRNA的剪接增强，推测第8节段也是如此，导致M2和NS2蛋白极度过量产生。血凝素（HA）裂解为HA1和HA2及其碳水化合物侧链的加工明显延迟且不完全。尽管一些HA被整合到质膜中，导致阳性血细胞吸附，但大部分HA积聚在靠近核膜的一个离散区室中，推测代表网状内皮和/或高尔基体网络。Vero细胞中的神经氨酸酶活性极低。核蛋白通常在感染后期从细胞核中释放。释放的感染性病毒极少，其传播受到高度阻碍。
