Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA.
Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA; BioSNTR, Brookings, SD 57007, USA.
Virology. 2019 Feb;528:152-163. doi: 10.1016/j.virol.2018.12.016. Epub 2019 Jan 5.
Influenza viruses are a group of respiratory pathogens that have evolved into four different types: A, B, C, and D. A common feature is that all four types are capable of replicating and transmitting among pigs. Here, we describe the development of isogenous cell culture system from the swine respiratory tract to study influenza viruses. Phenotypic characterization of swine primary nasal turbinate, trachea and lung cells revealed high expression of cytokeratin and demonstrated tissue site dependent expression of tight junction proteins. Furthermore, lectin binding assay on these cells demonstrated higher levels of Sia2-6Gal than Sia2-3Gal receptors and supported the replication of influenza A, B, C, and D viruses to appreciable levels at both 33 and 37 °C, but replication competence was dependent on virus type or temperature used. Overall, these swine primary respiratory cells showed epithelial phenotype, which is suitable for studying the comparative biology and pathobiology of influenza viruses.
流感病毒是一组呼吸道病原体，已经进化为 A、B、C 和 D 四种不同类型。一个共同的特点是，所有这四种类型都能够在猪之间复制和传播。在这里，我们描述了同源细胞培养系统的发展，该系统来自猪的呼吸道，用于研究流感病毒。猪原代鼻甲骨、气管和肺细胞的表型特征表明细胞角蛋白表达水平高，并表现出组织部位依赖的紧密连接蛋白表达。此外，对这些细胞进行的凝集素结合试验表明，Sia2-6Gal 受体的水平高于 Sia2-3Gal 受体，并且支持流感 A、B、C 和 D 病毒在 33 和 37°C 下都能达到可观的复制水平，但复制能力取决于病毒类型或使用的温度。总的来说，这些猪原代呼吸道细胞表现出上皮细胞表型，适合研究流感病毒的比较生物学和病理生物学。
