Friis N F, Szancer J
Danish Veterinary Laboratory, Copenhagen.
Acta Vet Scand. 1994;35(4):389-94. doi: 10.1186/BF03548313.
The sensitivity of some porcine and bovine mycoplasmas to potent antimicrobial agents was examined. Minimal inhibitory concentration (MIC) values were estimated for M. hyosynoviae, M. hyopneumoniae, M. dispar and M. bovis against enrofloxacin, lincomycin, tetracycline, tiamulin and tylosin, in a liquid medium test and in a disc assay. All 6 examined strains of each species and the respective type strains were significantly inhibited. The greatest sensitivity was noted for tiamulin against strains of M. hyosynoviae with a final MIC50 broth value of 0.025 micrograms ml-1 and disc value of 0.03 microgram per disc. Enrofloxacin was found very potent against M. hyopneumoniae with a final MIC50 of 0.025 microgram ml-1 and 0.1 microgram per disc, and for M. dispar with 0.05 microgram ml-1 and 0.03 microgram per disc. Most disc assay estimates in micrograms per disc were similar to or moderately greater than corresponding final broth figures in microgram ml-1. It may be possible to convert observed disc assay values into representative final broth MIC values for use in the clinic.
检测了一些猪和牛支原体对强效抗菌剂的敏感性。在液体培养基试验和纸片扩散试验中，评估了猪滑膜支原体、猪肺炎支原体、猪鼻支原体和牛支原体对恩诺沙星、林可霉素、四环素、替米考星和泰乐菌素的最低抑菌浓度（MIC）值。每个物种检测的所有6株菌株及相应的模式菌株均受到显著抑制。替米考星对猪滑膜支原体菌株的敏感性最高，最终肉汤MIC50值为0.025微克/毫升，纸片扩散法的值为每片0.03微克。发现恩诺沙星对猪肺炎支原体非常有效，最终MIC50为0.025微克/毫升和每片0.1微克，对猪鼻支原体则为0.05微克/毫升和每片0.03微克。大多数纸片扩散试验以微克/片为单位的估计值与相应的最终肉汤以微克/毫升为单位的数值相似或略高。有可能将观察到的纸片扩散试验值转换为用于临床的代表性最终肉汤MIC值。
