Lee T, Komiya T, Watanabe K, Aizawa C, Hashimoto H
Research Center for Biologicals, Kitasato Institute, Saitama, Japan.
Acta Virol. 1995 Apr;39(2):109-11.
E protein characteristics of the attenuated Japanese encephalitis (JE) virus strain SA(A) derived from the live vaccine strain SA14-14-2 were compared with those of the virulent strain SA(V). SA(A) showed lower haemagglutination (HA) activity with broader optimum pH range of HA reaction than SA(V), and the E protein of SA(A) had slower electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) than that of SA(V). These properties of SA(A) E protein appear to be caused by eight amino acid changes found in the E protein coding region of the SA(A) virus genome.
将源自活疫苗株SA14 - 14 - 2的减毒日本脑炎（JE）病毒株SA(A)的E蛋白特征与强毒株SA(V)的进行了比较。SA(A)的血凝（HA）活性较低，HA反应的最佳pH范围比SA(V)更宽，并且在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS - PAGE）中，SA(A)的E蛋白电泳迁移率比SA(V)的慢。SA(A) E蛋白的这些特性似乎是由SA(A)病毒基因组E蛋白编码区发现的8个氨基酸变化引起的。
