通过聚合酶链反应产物的限制性内切酶切割对人疱疹病毒6型DNA进行分型。
Typing of human herpesvirus-6 DNA by restriction endonuclease cleavage of the polymerase chain reaction product.
作者信息
Rajcáni J, Lábodyová A, Compel P, Roubalová K
机构信息
Institute of Virology, Slovak Academy of Sciences, Bratislava.
出版信息
Acta Virol. 1995 Apr;39(2):113-5.
PMID:7676936
Abstract
A 591 bp portion of the HindIII H (pZVH14) fragment of human herpesvirus-6 (HHV-6) strains GS (type A) and R-147 (type B) DNA was amplified by the polymerase chain reaction (PCR) using specific primers. While AluI cleaved the amplified DNA of both types of HHV-6, EcoRV cut the type A but not the type B DNA; vice versa, HaeIII cleaved the type B but not the type A DNA.
摘要
使用特异性引物通过聚合酶链反应（PCR）扩增人疱疹病毒6型（HHV-6）GS株（A型）和R-147株（B型）DNA的HindIII H（pZVH14）片段的591 bp部分。虽然AluI可切割两种类型HHV-6的扩增DNA，但EcoRV可切割A型而非B型DNA；反之，HaeIII可切割B型而非A型DNA。
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