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通过聚合酶链反应产物的限制性内切酶切割对人疱疹病毒6型DNA进行分型。

Typing of human herpesvirus-6 DNA by restriction endonuclease cleavage of the polymerase chain reaction product.

作者信息

Rajcáni J, Lábodyová A, Compel P, Roubalová K

机构信息

Institute of Virology, Slovak Academy of Sciences, Bratislava.

出版信息

Acta Virol. 1995 Apr;39(2):113-5.

PMID:7676936
Abstract

A 591 bp portion of the HindIII H (pZVH14) fragment of human herpesvirus-6 (HHV-6) strains GS (type A) and R-147 (type B) DNA was amplified by the polymerase chain reaction (PCR) using specific primers. While AluI cleaved the amplified DNA of both types of HHV-6, EcoRV cut the type A but not the type B DNA; vice versa, HaeIII cleaved the type B but not the type A DNA.

摘要

使用特异性引物通过聚合酶链反应(PCR)扩增人疱疹病毒6型(HHV-6)GS株(A型)和R-147株(B型)DNA的HindIII H(pZVH14)片段的591 bp部分。虽然AluI可切割两种类型HHV-6的扩增DNA,但EcoRV可切割A型而非B型DNA;反之,HaeIII可切割B型而非A型DNA。

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