McGuire T, Greally J, Leon J, Luka J
McGuire Clinic, Seat Pleasant, Maryland 20743, USA.
In Vivo. 1997 Jan-Feb;11(1):23-8.
DNA from Epstein-Barr virus (EBV) Types A, B and Herpesvirus-6 (HHV-6) Variants A and B was detected by the Polymerase chain reaction (PCR) in the saliva of 51 non-immunocompromised donor patients and in the blood of seventy non-immunocompromised donor patients with specific signs and symptoms. The minimum selection criteria for each patient included acute or recurrent upper respiratory infection, unilateral thoracolumbar muscle spasm and fatigue. PCR DNA detection in the saliva of selected donors revealed 80% of the donors had either Type A or B EBV (41 of 51), 34.1% Type B EBV only (14 of 41), 9% Type A only (4 of 41), and 56.1% Type A and B EBV (23 of 41). HHV-6 DNA was detected in 45.0% (23 of 51). PCR for EBV in blood of selected donors revealed 68.5% Type A or B EBV (48 of 70), 0% type B EBV alone, 64.8% Type A EBV only (31 of 48) and 35.4% both Type A and B EBV (17 of 48). HHV-6 was detected in 96.4% (64 of 70). The association of Type B EBV in the pathogenesis of these patients is explored based on the PCR quantitation of B type EBV DNA present in the samples.
通过聚合酶链反应（PCR）在51名无免疫功能低下的供体患者的唾液以及70名有特定体征和症状的无免疫功能低下的供体患者的血液中检测到了A型和B型爱泼斯坦-巴尔病毒（EBV）以及A、B型6型疱疹病毒（HHV-6）。每位患者的最低入选标准包括急性或复发性上呼吸道感染、单侧胸腰段肌肉痉挛和疲劳。对选定供体唾液进行的PCR DNA检测显示，80%的供体感染了A型或B型EBV（51例中的41例），仅感染B型EBV的占34.1%（41例中的14例），仅感染A型的占9%（41例中的4例），同时感染A型和B型EBV的占56.1%（41例中的23例）。在45.0%（51例中的23例）的样本中检测到了HHV-6 DNA。对选定供体血液进行的EBV PCR检测显示，68.5%的样本感染了A型或B型EBV（70例中的48例），单独感染B型EBV的比例为0%，仅感染A型EBV的占64.8%（48例中的31例），同时感染A型和B型EBV的占35.4%（48例中的17例）。在96.4%（70例中的64例）的样本中检测到了HHV-6。基于样本中B型EBV DNA的PCR定量分析，探讨了B型EBV在这些患者发病机制中的作用。
