Experimental transmission of eastern equine encephalitis virus and Highlands J virus via semen of infected tom turkeys.

Tom turkeys were experimentally inoculated with eastern equine encephalitis (EEE) virus or Highlands J (HJ) virus; semen was examined for presence of virus and ability to transmit infection by artificial insemination. Mild depression and inappetence were observed in tom turkeys inoculated with either EEE virus or HJ virus. Toms were viremic on days 1-2 postinoculation (PI), and virus was shed in semen on days 1-5 PI. Semen collected from EEE-virus-inoculated or HJ-virus-inoculated toms on days 1-2 PI and inseminated into turkey breeder hens transmitted the infection. EEE virus was detected in one of 10 hens after insemination with semen from EEE-virus-inoculated toms, and HJ virus was detected in three of 10 hens after insemination with semen from HJ-virus-inoculated toms. These results indicate that semen is a potential vehicle for transmission of EEE virus and HJ virus.