Tsujimura A, Yasojima K, Kuboki Y, Suzuki A, Ueno N, Shiokawa K, Hashimoto-Gotoh T
Department of Biochemistry and Molecular Genetics, Kyoto Prefectural University of Medicine, Japan.
Biochem Biophys Res Commun. 1995 Sep 14;214(2):432-9. doi: 10.1006/bbrc.1995.2305.
Using conserved nucleotide sequences in mammalian osteoblast specific factor-1 (OSF-1) coding regions, we isolated two kinds of cDNA clones from the Xenopus brain library. The encoded proteins, named Xenopus pleiotrophic factors (X-PTFs)-alpha and -beta, were 65 and 87% homologous to human midkine and OSF-1, respectively. In the adult frog, X-ptf-alpha was expressed in the ovary, brain, eye, bone, heart and lung, whereas X-ptf-beta was expressed in the brain, eye and bone. By in situ hybridization of the tailbud embryo, X-ptf-alpha mRNA was detected rather broadly in the head/tail regions including the central nervous system (CNS), whereas X-ptf-beta mRNA was restricted to the CNS, particularly in the hind-brain. During embryogenesis, X-ptf-alpha mRNA was detected in the one-cell stage embryo, whereas only zygotic expression was observed in X-ptf-beta. X-ptf-beta mRNAs contained approximately 79 bp tandem repeats in the 3'-untranslated region, complementary to those found in retinoic acid cellular receptor mRNA and in the sense strand of short interspersed repeat transcripts in X. laevis.
利用哺乳动物成骨细胞特异性因子-1（OSF-1）编码区的保守核苷酸序列，我们从非洲爪蟾脑文库中分离出两种cDNA克隆。所编码的蛋白质分别命名为非洲爪蟾多效因子（X-PTFs）-α和-β，它们与人中期因子和OSF-1的同源性分别为65%和87%。在成年蛙中，X-ptf-α在卵巢、脑、眼、骨、心脏和肺中表达，而X-ptf-β在脑、眼和骨中表达。通过对尾芽胚胎进行原位杂交，在包括中枢神经系统（CNS）在内的头/尾区域广泛检测到X-ptf-α mRNA，而X-ptf-β mRNA局限于中枢神经系统，特别是在后脑。在胚胎发育过程中，在单细胞期胚胎中检测到X-ptf-α mRNA，而在X-ptf-β中仅观察到合子表达。X-ptf-β mRNA在3'-非翻译区含有约79 bp的串联重复序列，与维甲酸细胞受体mRNA以及非洲爪蟾短散在重复转录本的有义链中发现的序列互补。
