鼠伤寒沙门氏菌菌株YG1024和YG1012检测芳香胺和硝基芳烃致突变性的敏感性比较。

Comparison of the sensitivity of Salmonella typhimurium strains YG1024 and YG1012 for detecting the mutagenicity of aromatic amines and nitroarenes.

作者信息

Watanabe M, Sofuni T, Nohmi T

机构信息

Division of Genetics and Mutagenesis, National Institute of Hygienic Sciences, Tokyo, Japan.

出版信息

Mutat Res. 1993 Jan;301(1):7-12. doi: 10.1016/0165-7992(93)90049-2.

DOI:10.1016/0165-7992(93)90049-2

PMID:7677947

Abstract

Salmonella typhimurium YG1024 is a derivative of S. typhimurium TA98 with a high level of N-hydroxyarylamine O-acetyltransferase (OAT) activity. We have demonstrated that this strain is highly sensitive to the mutagenic actions of N-hydroxyarylamines derived from aromatic amines and nitroarenes. In this paper, we compared the sensitivities of YG1024 with those of S. typhimurium YG1012, which has about 4 times higher OAT activity than YG1024 but lacks plasmid pKM101. It turned out that YG1024 was more sensitive to the mutagenic actions of 1-aminonaphthalene, 1-nitropyrene, 1,8-dinitropyrene and 2-nitronaphthalene than YG1012 and showed comparable sensitivity to 2-hydroxy-acetylaminofluorene, 2-aminoanthracene and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) to YG1012. These results suggested that YG1024 is more suitable than YG1012 for the efficient detection of mutagenic aromatic amines and nitroarenes.

摘要

鼠伤寒沙门氏菌YG1024是鼠伤寒沙门氏菌TA98的衍生物，具有高水平的N-羟基芳胺O-乙酰转移酶（OAT）活性。我们已经证明，该菌株对源自芳香胺和硝基芳烃的N-羟基芳胺的诱变作用高度敏感。在本文中，我们比较了YG1024与鼠伤寒沙门氏菌YG1012的敏感性，YG1012的OAT活性比YG1024高约4倍，但缺乏质粒pKM101。结果表明，YG1024对1-氨基萘、1-硝基芘、1,8-二硝基芘和2-硝基萘的诱变作用比YG1012更敏感，并且对2-羟基乙酰氨基芴、2-氨基蒽和2-氨基-6-甲基二吡啶并[1,2-a:3',2'-d]咪唑（Glu-P-1）的敏感性与YG1012相当。这些结果表明，YG1024比YG1012更适合用于高效检测诱变芳香胺和硝基芳烃。