Connolly K M, Bogdanffy M S
E.I. du Pont de Nemours and Co., Haskell Laboratory for Toxicology and Industrial Medicine, Newark, DE 19714.
J Histochem Cytochem. 1993 Jan;41(1):1-6. doi: 10.1177/41.1.7678022.
Proliferating cell nuclear antigen (PCNA) was evaluated as a marker of cell proliferation in formalin-fixed rat liver tissue through a comparative study with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU). The comparison was conducted through the introduction of a dual immunohistochemical procedure that allows the simultaneous detection of the two antigens. The results of this study suggest that although statistically similar indexes for each can be achieved, what has been reported to be the "S-phase fraction" of PCNA-labeled nuclei is significantly different from the population of cells marked by BrdU. The data also suggest that the reason for this difference is that the "S-phase fraction" of PCNA-labeled nuclei is the population of cells in late G1- and early S-phases. BrdU, by comparison, is incorporated into cells only during DNA synthesis. Therefore, although BrdU and PCNA labeling techniques may both be effective for evaluating cell proliferation rates, it must be recognized that labeling indices derived from each are not entirely synonymous. The method presented here for the simultaneous labeling of PCNA and BrdU antigens may have utility in studies of cell cycle perturbations.
通过与胸腺嘧啶类似物5-溴-2'-脱氧尿苷（BrdU）进行比较研究，评估增殖细胞核抗原（PCNA）作为福尔马林固定大鼠肝脏组织中细胞增殖的标志物。通过引入一种双免疫组织化学程序进行比较，该程序允许同时检测这两种抗原。这项研究的结果表明，尽管每种抗原在统计学上可以获得相似的指标，但据报道的PCNA标记细胞核的“S期分数”与BrdU标记的细胞群体有显著差异。数据还表明，这种差异的原因是PCNA标记细胞核的“S期分数”是处于G1晚期和S期早期的细胞群体。相比之下，BrdU仅在DNA合成期间掺入细胞。因此，尽管BrdU和PCNA标记技术都可能有效地评估细胞增殖率，但必须认识到，从每种技术得出的标记指数并不完全同义。本文介绍的同时标记PCNA和BrdU抗原的方法可能在细胞周期扰动研究中有用。
