Takada A, Ohmori K, Yoneda T, Tsuyuoka K, Hasegawa A, Kiso M, Kannagi R
Laboratory of Experimental Pathology, Aichi Cancer Center, Nagoya, Japan.
Cancer Res. 1993 Jan 15;53(2):354-61.
The carbohydrate antigen, sialyl Lex, is known to be a ligand for the cell adhesion molecule called ELAM-1 (E-selectin, endothelial cell leukocyte adhesion molecule-1), which is present on cytokine-activated human endothelial cells. Recently, we reported that another carbohydrate antigen, sialyl Lea, can also serve as a ligand for ELAM-1 (A. Takada, K. Ohmori, N. Takahashi, K. Tsuyuoka, K. Yago, K. Zenita, A. Hasegawa, and R. Kannagi, Biochem. Biophys. Res. Commun., 179: 713-719, 1991). Both sialyl Lex and sialyl Lea are expressed in many human malignant cells. In order to assess the contribution of these carbohydrate antigens to the adhesion of human malignant cells to vascular endothelium, we selected a panel of 12 cultured human epithelial cancer cell lines and a panel of 12 human leukemia cell lines which express sialyl Lex and/or sialyl Lea antigens. All 12 epithelial cancer cell lines exhibited a clearly ELAM-1-dependent adhesion to cytokine-activated human umbilical vein endothelial cells, while only 3 of the 12 leukemia cell lines exhibited significant participation of ELAM-1 in the adhesion. With regard to epithelial cancer cells, the adhesion of 6 cancer cell lines, mostly of colon and pancreas origin, was dependent almost exclusively on sialyl Lea. A significant contribution of the sialyl Lex antigen was noted in the adhesion of the other 6 cell lines, including cancers of lung and liver origin. These results imply that the sialyl Lea/ELAM-1 adhesion system, as well as the sialyl Lex/ELAM-1 adhesion system, plays an important role in the adhesion of human cancer cells to human umbilical vein endothelial cells. With regard to leukemia cells, on the other hand, adhesion of the 3 leukemia cell lines that showed ELAM-1-dependent adhesion was mediated by the sialyl Lex antigen, and none of these leukemia cell lines expressed sialyl Lea or exhibited sialyl Lea-dependent adhesion.
碳水化合物抗原唾液酸化路易斯（sialyl Lex）是细胞黏附分子ELAM-1（E-选择素，内皮细胞白细胞黏附分子-1）的配体，ELAM-1存在于细胞因子激活的人内皮细胞上。最近，我们报道了另一种碳水化合物抗原唾液酸化莱阿（sialyl Lea）也可作为ELAM-1的配体（A. 高田、大森健、高桥直、角冈克、矢后和、禅田克、长谷川明、金波仁，《生物化学与生物物理学研究通讯》，179: 713 - 719, 1991）。唾液酸化路易斯和唾液酸化莱阿在许多人类恶性细胞中均有表达。为了评估这些碳水化合物抗原对人类恶性细胞与血管内皮细胞黏附的作用，我们挑选了一组12种培养的人上皮癌细胞系和一组12种人白血病细胞系，它们表达唾液酸化路易斯和/或唾液酸化莱阿抗原。所有12种上皮癌细胞系对细胞因子激活的人脐静脉内皮细胞均表现出明显的依赖ELAM-1的黏附，而12种白血病细胞系中只有3种在黏附中表现出ELAM-1的显著参与。对于上皮癌细胞，6种癌细胞系的黏附，大多起源于结肠和胰腺，几乎完全依赖于唾液酸化莱阿。在包括起源于肺和肝的其他6种细胞系的黏附中，注意到唾液酸化路易斯抗原的显著作用。这些结果表明，唾液酸化莱阿/ELAM-1黏附系统以及唾液酸化路易斯/ELAM-1黏附系统在人类癌细胞与人脐静脉内皮细胞的黏附中起重要作用。另一方面，对于白血病细胞，表现出依赖ELAM-1黏附的3种白血病细胞系的黏附是由唾液酸化路易斯抗原介导的，这些白血病细胞系均不表达唾液酸化莱阿，也未表现出依赖唾液酸化莱阿的黏附。
