在呼吸道合胞病毒附着（G）蛋白基因衍生克隆的受限区域中观察到频繁的聚合酶错误。
Frequent polymerase errors observed in a restricted area of clones derived from the attachment (G) protein gene of respiratory syncytial virus.
作者信息
Cane P A, Matthews D A, Pringle C R
机构信息
Department of Biological Sciences, University of Warwick, Coventry, United Kingdom.
出版信息
J Virol. 1993 Feb;67(2):1090-3. doi: 10.1128/JVI.67.2.1090-1093.1993.
Abstract
Sequence analysis of a large number of clones derived from the carboxy-terminal one-third of the attachment (G) protein gene of subgroup A respiratory syncytial viruses revealed a region very prone to polymerase errors which resulted mainly in frameshifts because of the insertion or deletion of adenosine residues in some but not all runs of such residues. Such mutations were detected in 14% of clones derived from mRNA, 58% of clones derived from genomic-sense RNA, and 50% of clones derived from in vitro-transcribed RNA. This phenomenon appears to be dependent on the template sequence.
摘要
对大量来自A亚群呼吸道合胞病毒黏附（G）蛋白基因羧基末端三分之一区域的克隆进行序列分析，发现一个极易发生聚合酶错误的区域，这种错误主要导致移码，原因是在部分（而非全部）此类残基的连续排列中插入或缺失了腺苷残基。在源自mRNA的克隆中有14%检测到此类突变，源自基因组正义RNA的克隆中有58%检测到此类突变，源自体外转录RNA的克隆中有50%检测到此类突变。这种现象似乎取决于模板序列。
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