Thomas S M, Lamb R A, Paterson R G
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208.
Cell. 1988 Sep 9;54(6):891-902. doi: 10.1016/s0092-8674(88)91285-8.
The "P" gene of the paramyxovirus SV5 encodes two known proteins, P (Mr approximately equal to 44,000) and V (Mr approximately equal to 24,000). The complete nucleotide sequence of the "P" gene has been obtained and is found to contain two open reading frames, neither of which is large enough to encode the P protein. We have shown that the P and V proteins are translated from two mRNAs that differ by the presence of two nontemplated G residues in the P mRNA. These two additional nucleotides convert the two open reading frames to one of 392 amino acids. The P and V proteins are amino coterminal and have 164 amino acids in common. The unique C terminus of V consists of a cysteine-rich region that resembles a cysteine-rich metal binding domain. An open reading frame that contains this cysteine-rich region exists in all other paramyxovirus "P" gene sequences examined, which suggests that it may have important biological significance.
副粘病毒SV5的“P”基因编码两种已知蛋白，即P（分子量约为44,000）和V（分子量约为24,000）。已获得“P”基因的完整核苷酸序列，发现其包含两个开放阅读框，但均不足以编码P蛋白。我们已表明，P蛋白和V蛋白由两种mRNA翻译而来，这两种mRNA的区别在于P mRNA中存在两个非模板化的G残基。这两个额外的核苷酸将两个开放阅读框转变为一个含392个氨基酸的阅读框。P蛋白和V蛋白的氨基末端相同，共有164个氨基酸。V蛋白独特的C末端由一个富含半胱氨酸的区域组成，该区域类似于富含半胱氨酸的金属结合结构域。在所有其他已检测的副粘病毒“P”基因序列中均存在一个包含此富含半胱氨酸区域的开放阅读框，这表明它可能具有重要的生物学意义。
