Wineman J P, Nishikawa S, Müller-Sieburg C E
Medical Biology Institute, La Jolla, CA 92037.
Blood. 1993 Jan 15;81(2):365-72.
We show here that mouse pluripotent hematopoietic stem cells can be maintained in vitro on stroma for at least 3 weeks at levels close to those found in bone marrow. The extent of stem cell maintenance is affected by the nature of the stromal cells. The stromal cell line S17 supported stem cells significantly better than heterogeneous, primary stromal layers or the stromal cell line Strofl-1. Stem cells cultured on S17 repopulated all hematopoietic lineages in marrow-ablated hosts for at least 10 months, indicating that this culture system maintained primitive stem cells with extensive proliferative capacity. Furthermore, we demonstrate that, while pluripotent stem cells express c-kit, this receptor appears to play only a minor role in stem cell maintenance in vitro. The addition of an antibody that blocks the interaction of c-kit with its ligand essentially abrogated myelopoiesis in cultures. However, the level of stem cells in antibody-treated cultures was similar to that found in untreated cultures. Thus, it seems likely that the maintenance of primitive stem cells in vitro depends on yet unidentified stromal cell-derived factor(s).
我们在此表明，小鼠多能造血干细胞可在体外基质上维持至少3周，其水平接近骨髓中的水平。干细胞维持的程度受基质细胞性质的影响。基质细胞系S17比异质性原代基质层或基质细胞系Strofl-1能更好地支持干细胞。在S17上培养的干细胞在骨髓消融宿主中重新填充所有造血谱系至少10个月，表明该培养系统维持了具有广泛增殖能力的原始干细胞。此外，我们证明，虽然多能干细胞表达c-kit，但该受体在体外干细胞维持中似乎仅起次要作用。添加阻断c-kit与其配体相互作用的抗体基本上消除了培养物中的髓系造血。然而，抗体处理培养物中的干细胞水平与未处理培养物中的相似。因此，体外原始干细胞的维持似乎依赖于尚未确定的基质细胞衍生因子。
