Evans H F, Huntley G W, Morrison J H, Shine J
Garvan Institute of Medical Research, St. Vincent's Hospital, Sydney, New South Wales, Australia.
J Comp Neurol. 1993 Feb 8;328(2):203-12. doi: 10.1002/cne.903280204.
The hypothalamic and basal forebrain sites of synthesis of preprogalanin mRNA were identified in three adult monkeys (Macaca fascicularis) by in situ hybridisation performed with a radiolabelled cRNA probe transcribed from human preprogalanin cDNA. With stringent hybridisation conditions, the cRNA probe was hybridised to free-floating sections containing structures contiguous with the rostral hypothalamus through to the caudal limit of the hypothalamus as defined by the mammillary bodies. Specific hybridisation of the preprogalanin cRNA probe occurred throughout the hypothalamus but was particularly intense in the arcuate, paraventricular (parvicellular and magnocellular portions), and dorsomedial nuclei. Moderate hybridisation was found in the periventricular nucleus and scattered hybridisation in the medial preoptic nucleus. The medial preoptic area and the anterior and lateral hypothalamic areas showed moderate to intense hybridisation in scattered cells. A few cells in the tuberal portion and dorsal cap of the anterior portion of the supraoptic nucleus were labelled. Isolated cells were also labelled in the zona incerta. There was little labelling in the dorsal hypothalamic area but moderate labelling in the posterior hypothalamic area. Structures contiguous with the rostral hypothalamus including the diagonal band of Broca, bed nucleus of stria terminalis, substantia innominata, and basal nucleus of Meynert showed intense hybridisation. These data indicate a widespread distribution of preprogalanin mRNA in the monkey hypothalamus. A comparison with the previously reported distribution of preprogalanin mRNA in the rat, as well as with the distribution of galanin-like immunoreactivity in the rat and human, suggests some important species differences. Of particular interest were differences in the supraoptic, suprachiasmatic, and dorsomedial nuclei. The intense hybridisation throughout the paraventricular nucleus and in the rostral arcuate nucleus suggests that galanin may play a role in the regulation of both posterior and anterior pituitary function.
通过使用从人前甘丙肽cDNA转录的放射性标记cRNA探针进行原位杂交，在三只成年恒河猴（食蟹猴）中确定了前甘丙肽原mRNA的下丘脑和基底前脑合成位点。在严格的杂交条件下，cRNA探针与游离切片杂交，这些切片包含与下丘脑前部直至乳头体所定义的下丘脑尾端界限连续的结构。前甘丙肽原cRNA探针的特异性杂交在下丘脑各处均有发生，但在弓状核、室旁核（小细胞部和大细胞部）以及背内侧核中尤为强烈。在室周核中发现中等程度的杂交，在内侧视前核中有散在的杂交。内侧视前区以及下丘脑前区和外侧区在散在细胞中显示中等至强烈的杂交。视上核前部的结节部和背帽中有少数细胞被标记。未定带中也有孤立的细胞被标记。下丘脑背侧区标记很少，但下丘脑后区有中等程度的标记。与下丘脑前部连续的结构，包括布洛卡斜带、终纹床核、无名质和迈内特基底核显示强烈杂交。这些数据表明前甘丙肽原mRNA在猴下丘脑中分布广泛。与先前报道的大鼠前甘丙肽原mRNA分布以及大鼠和人类中甘丙肽样免疫反应性分布的比较表明存在一些重要的物种差异。特别有趣的是视上核、视交叉上核和背内侧核中的差异。室旁核和弓状核前部的强烈杂交表明甘丙肽可能在调节垂体后叶和垂体前叶功能中起作用。
