Oberbauer A M, Strong D D, Linkhart T A, Mohan S, Longo L D
Department of Animal Science, University of California, Davis 95616-8521.
Endocrinology. 1993 Feb;132(2):757-62. doi: 10.1210/endo.132.2.7678798.
In previous studies, we found that basic fibroblast growth factor (bFGF) significantly stimulated the secretion of hCG beta in the Jar choriocarcinoma cell line. In the present study, the effect of bFGF on the steady state hCG beta mRNA level in this cell line was determined. Application of Northern analyses with total RNA isolated from bFGF-stimulated Jar cells revealed that, in a time-dependent manner, the steady state hCG beta mRNA level increased progressively, reaching 4-fold of the control value within 4 h after exposure to bFGF. The observed accumulation was due in part to increased transcription (2.4-fold relative to that in control cultures), as determined by nuclear transcription studies. In addition, bFGF increased the stability of the hCG beta message; the message half-life was increased from approximately 3 h (in control cultures) to greater than 6 h (in bFGF-treated cultures). These data demonstrate that bFGF stimulates hCG beta mRNA accumulation in a complex manner regulated through both transcriptional and posttranscriptional mechanisms.
在先前的研究中，我们发现碱性成纤维细胞生长因子（bFGF）能显著刺激Jar绒毛膜癌细胞系中hCGβ的分泌。在本研究中，我们测定了bFGF对该细胞系中hCGβ mRNA稳态水平的影响。对从bFGF刺激的Jar细胞中分离的总RNA进行Northern分析表明，hCGβ mRNA稳态水平以时间依赖性方式逐渐升高，在暴露于bFGF后4小时内达到对照值的4倍。通过细胞核转录研究确定，观察到的积累部分归因于转录增加（相对于对照培养物增加2.4倍）。此外，bFGF增加了hCGβ信息的稳定性；信息半衰期从大约3小时（对照培养物中）增加到大于6小时（bFGF处理的培养物中）。这些数据表明，bFGF通过转录和转录后机制以复杂的方式刺激hCGβ mRNA的积累。
