Proudlock F, Spike R C, Todd A J
Department of Anatomy, University of Glasgow, Scotland.
J Comp Neurol. 1993 Jan 8;327(2):289-97. doi: 10.1002/cne.903270210.
In order to determine whether somatostatin coexists with GABA or glycine in neurones in rat spinal dorsal horn, a combined pre- and post-embedding immunocytochemical study was carried out. One hundred six somatostatin-immunoreactive neurones located in lamina II and the dorsal half of lamina III were tested with antiserum or monoclonal antibody to GABA and none of these cells showed GABA-like immunoreactivity. However, 8 out of 13 somatostatin-immunoreactive neurones located deeper in the dorsal horn (ventral lamina III and lamina IV) showed glycine-like immunoreactivity, and 6 of these were also GABA-immunoreactive. We have previously shown that neurotensin-immunoreactive neurones in laminae II and III are also not immunoreactive when tested with GABA antiserum (Todd et al.: Neuroscience 47:685-691, 1992), and a double-labelling fluorescence method was therefore used to compare the distribution of somatostatin and neurotensin within the superficial dorsal horn. The two types of peptide-immunoreactivity were never found in the same profile. These results suggest that somatostatin and neurotensin are present in different populations of non-GABAergic neurones in rat superficial dorsal horn, but that some somatostatin-containing neurones in the deeper part of the dorsal horn contain glycine, with or without GABA.
为了确定生长抑素是否与γ-氨基丁酸（GABA）或甘氨酸在大鼠脊髓背角神经元中共存，进行了一项包埋前和包埋后联合免疫细胞化学研究。对位于Ⅱ层和Ⅲ层背侧半层的106个生长抑素免疫反应性神经元用抗GABA抗血清或单克隆抗体进行检测，这些细胞均未显示出GABA样免疫反应性。然而，位于背角更深部位（Ⅲ层腹侧和Ⅳ层）的13个生长抑素免疫反应性神经元中有8个显示出甘氨酸样免疫反应性，其中6个也有GABA免疫反应性。我们之前已经表明，当用GABA抗血清检测时，Ⅱ层和Ⅲ层中的神经降压素免疫反应性神经元也没有免疫反应性（托德等人：《神经科学》47:685 - 691, 1992），因此使用双标记荧光法比较生长抑素和神经降压素在脊髓背角浅层的分布。这两种肽免疫反应性从未在同一细胞轮廓中发现。这些结果表明，生长抑素和神经降压素存在于大鼠脊髓背角浅层不同的非GABA能神经元群体中，但背角深部一些含生长抑素的神经元含有甘氨酸，有的还含有GABA。
