Todd A J, Sullivan A C
Department of Anatomy, University of Glasgow, United Kingdom.
J Comp Neurol. 1990 Jun 15;296(3):496-505. doi: 10.1002/cne.902960312.
The distributions of GABA-like and glycine-like immunoreactivities in the rat spinal cord were compared by using postembedding immunohistochemistry on semithin sections. In laminae I, II, and III, the proportions of GABA immunoreactive cells were 28%, 31%, and 46%, respectively, whereas for glycine immunoreactive cells the proportions were 9%, 14%, and 30%. Nearly all of the glycine immunoreactive cells in this area were also immunoreactive with the anti-GABA antiserum. In lamina II, some Golgi-stained islet cells were glycine immunoreactive, whereas others were not. Immunoreactive cell bodies were also present in the remainder of the grey matter. Some of these reacted with anti-GABA or antiglycine antiserum; others showed immunoreactivity with both antisera. Immunoreactive axons were found in the ventral and lateral funiculi of the white matter. Many large axons reacted with antiglycine antiserum, whereas GABA-immunoreactive axons were mostly of small diameter. Some large and small axons showed both types of immunoreactivity. These results suggest that the inhibitory neurotransmitters GABA and glycine coexist within cell bodies and axons in the rat spinal cord.
通过对半薄切片进行包埋后免疫组织化学方法，比较了大鼠脊髓中γ-氨基丁酸（GABA）样和甘氨酸样免疫反应性的分布。在Ⅰ、Ⅱ和Ⅲ层中，GABA免疫反应性细胞的比例分别为28%、31%和46%，而甘氨酸免疫反应性细胞的比例分别为9%、14%和30%。该区域几乎所有的甘氨酸免疫反应性细胞也与抗GABA抗血清发生免疫反应。在Ⅱ层中，一些经高尔基染色的胰岛细胞是甘氨酸免疫反应性的，而另一些则不是。免疫反应性细胞体也存在于灰质的其余部分。其中一些与抗GABA或抗甘氨酸抗血清发生反应；另一些则对两种抗血清均表现出免疫反应性。在白质的腹侧和外侧索中发现了免疫反应性轴突。许多大的轴突与抗甘氨酸抗血清发生反应，而GABA免疫反应性轴突大多直径较小。一些大的和小的轴突表现出两种类型的免疫反应性。这些结果表明，抑制性神经递质GABA和甘氨酸在大鼠脊髓的细胞体和轴突中共存。
