Shah I A, Netto D, Schlageter M O, Muth C, Fox I, Manne R K
Department of Laboratory Service, Veterans Affairs Medical Centers, Phoenix, Arizona.
Mod Pathol. 1993 Jan;6(1):3-9.
To differentiate Merkel-cell tumor (MCT) from other neuroendocrine (NE) carcinomas, we immunostained (using avidin-biotin-peroxidase method) nine MCT and 37 NE (including 28 small-cell) carcinomas for NE markers (neuron-specific enolase and chromogranin), cytokeratin, neurofilament, vimentin, and a number of other markers. Cytokeratin was positive in 100% of MCT and in 85% of small-cell carcinomas; neurofilament and vimentin were positive in respectively 100% and 22% of MCT and 0% of NE carcinomas. Our data suggest that the coexpression of cytokeratin and neurofilament by an undifferentiated dermal or visceral tumor is of significant help in diagnosing MCT and differentiating it from small-cell carcinomas. The vimentin reactivity is a weak and insignificant discriminant.
为了将默克尔细胞癌（MCT）与其他神经内分泌（NE）癌区分开来，我们（采用抗生物素蛋白-生物素-过氧化物酶法）对9例MCT和37例NE癌（包括28例小细胞癌）进行免疫染色，检测NE标志物（神经元特异性烯醇化酶和嗜铬粒蛋白）、细胞角蛋白、神经丝、波形蛋白以及其他一些标志物。细胞角蛋白在100%的MCT和85%的小细胞癌中呈阳性；神经丝和波形蛋白在MCT中的阳性率分别为100%和22%，而在NE癌中的阳性率为0%。我们的数据表明，未分化的皮肤或内脏肿瘤中细胞角蛋白和神经丝的共表达对诊断MCT并将其与小细胞癌区分开来有显著帮助。波形蛋白反应性是一个微弱且无显著意义的鉴别指标。
