Boag A H, Young I D
Department of Pathology, Kingston General Hospital, Ontario, Canada.
Mod Pathol. 1993 Jan;6(1):65-8.
The 72-kDa Type IV collagenase (T4C) is a matrix metalloproteinase, the expression of which may be important in normal basement membrane metabolism. The production of T4C by malignant cells has been linked to their invasive and metastatic potential in several tumor systems. The pattern of T4C immunoreactivity was assessed in formalin-fixed, paraffin-embedded prostatic tissue using polyclonal, monospecific antibodies. Basal cells in normal and hyperplastic epithelium demonstrated slight to moderate cytoplasmic immunoreactivity, while secretory epithelial cells showed generally weaker immunostaining. In 35 of 35 cases of primary adenocarcinoma and five of five cases of metastatic adenocarcinoma in lymph nodes, the majority of malignant cells showed strong immunoreactivity. Similar strong immunostaining was also seen in foci of prostatic intraepithelial neoplasia. The high level of expression of T4C in prostatic adenocarcinoma and its metastases suggests this metalloproteinase may play a role in determining the invasive and metastatic properties of this tumor. The enhanced immunoreactivity in prostatic intraepithelial neoplasia suggests the induction of T4C may be an early event in the development of the invasive phenotype. The expression of T4C in benign epithelial cells may be a manifestation of its putative physiological role in basement membrane metabolism.
72-kDa IV型胶原酶（T4C）是一种基质金属蛋白酶，其表达在正常基底膜代谢中可能很重要。在多个肿瘤系统中，恶性细胞产生T4C与其侵袭和转移潜能有关。使用多克隆单特异性抗体对福尔马林固定、石蜡包埋的前列腺组织中T4C免疫反应性模式进行了评估。正常和增生上皮中的基底细胞表现出轻微至中度的细胞质免疫反应性，而分泌上皮细胞的免疫染色通常较弱。在35例原发性腺癌和5例淋巴结转移性腺癌中，大多数恶性细胞表现出强免疫反应性。在前列腺上皮内瘤变灶中也观察到类似的强免疫染色。T4C在前列腺腺癌及其转移灶中的高表达表明这种金属蛋白酶可能在决定该肿瘤的侵袭和转移特性中起作用。前列腺上皮内瘤变中增强的免疫反应性表明T4C的诱导可能是侵袭性表型发展中的早期事件。T4C在良性上皮细胞中的表达可能是其在基底膜代谢中假定生理作用的一种表现。
