Lazcano O, Speights V O, Strickler J G, Bilbao J E, Becker J, Diaz J
Section of Surgical Pathology, Mayo Clinic, Rochester, Minnesota.
Mod Pathol. 1993 Jan;6(1):80-4.
Three combinations of histochemical stains were used to study 69 routinely processed tissues containing various "yeast-like" fungal organisms (Cryptococcus neoformans, Blastomyces dermatitidis, Coccidioides immitis, Histoplasma capsulatum, Paracoccidioides brasiliensis, and Candida albicans). Fontana-Masson stain combined either with mucicarmine or Alcian blue produced distinctive staining of C. neoformans (Fontana-Masson-positive wall and mucin-positive capsule) that was not identified in other fungi. The Alcian blue stain combined with periodic acid-Schiff reaction also was useful for fungal classification, because the Alcian blue component reacted with only C. neoformans (capsule) and B. dermatitidis (wall) and the periodic acid-Schiff reaction reacted with the cell wall and body of each fungus. We concluded that the combined histochemical stains used in conjunction with morphologic study of hematoxylin and eosin- and Gomori methenamine silver-stained sections are helpful in the differential diagnosis of yeast-like fungal organisms.
使用三种组织化学染色组合，对69份常规处理的组织进行研究，这些组织含有各种“酵母样”真菌（新型隐球菌、皮炎芽生菌、粗球孢子菌、荚膜组织胞浆菌、巴西副球孢子菌和白色念珠菌）。Fontana-Masson染色与黏液卡红或阿尔辛蓝联合使用，可对新型隐球菌产生独特染色（Fontana-Masson阳性壁和黏液阳性荚膜），这在其他真菌中未发现。阿尔辛蓝染色与过碘酸-希夫反应联合使用，对真菌分类也很有用，因为阿尔辛蓝成分仅与新型隐球菌（荚膜）和皮炎芽生菌（壁）反应，而过碘酸-希夫反应与每种真菌的细胞壁和菌体反应。我们得出结论，联合组织化学染色与苏木精和伊红及Gomori六胺银染色切片的形态学研究相结合，有助于酵母样真菌的鉴别诊断。
